Product DescriptionAdditional InformationData & FiguresTechnical LibraryReference LiteratureDownloadsPromoCell’s crypreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in PromoCell’s well-proven M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using PromoCells’s proprietary, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial contains more than 1.5 million or 5 million viable cells after thawing, respectively.NEW: Our M2 Macrophages are now also available from HLA-typed donors.Recommended plating density200,000 cells per cm2Tested MarkersCD163 positive, CD68 positiveFigure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.Figure 1. Culture of PromoCell"s cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay.CloseFigure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).Figure 2. Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macrophages exhibit a CD68+ (99,74%) and CD80+ (83,33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99,72%) and CD163+ (94,91%) marker expression profile, typical for M2 macrophages (lower row).Close