Product overview Technical/Spec Support/Documents FAQs Tips for usage Related products Description ExcelTaq™ Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.Features5"→3" DNA polymerase activity3"→5" exonuclease activity (proofreading)Thermo-stable up to 98°C during PCR denaturing step4× fidelity as compared to Taq DNA polymeraseRobust PCR performance, resistance to variance in PCR condition Storage-20°C for 24 months Elongation capabilityExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from λDNA up to 12 kb (M: DM5100).   Sensitivity ExcelTaq™ Klen-Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM2100).     Contents ComponentVolume Klen-Taq DNA Polymerase (5 U/μl)100 μl 10X Klen Buffer1.2 ml ExcelTaq™ Klen-Taq DNA Polymerase mixtureKlen-Taq DNA polymerase             5 U/ µl A proofreading DNA polymerase   Trace Storage Buffer40 mM Tris-HCl (pH 7.5), 50 mM KCl, 25 mM (NH4)2SO4, 0.1 mM EDTA, 5.0 mM 2-mercaptoethanol, 50% (v/v) glycerol10X Klen Buffer400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. Storage-20°C for 24 months  ManualManual_TK1000_ExcelTaq™ Klen-Taq DNA PolymeraseSDSSDS_TK1000 General Terms and Conditions / Service Policies Does  ExcelTaq™ Klen-Taq DNA Polymerase  (TK1000) product have chemical modification or biological modification?It is without any modification. Moreover, the proof-reading activity of Klen-taq products is thrice that of a normal Taq DNA polymerase. Is ExcelTaq™ Klen-Taq DNA Polymerase (TK1000) a blended enzyme mix? Klen-Taq DNA polymerase (TK1000) is blended KlenTaq DNA polymerse and a small amount proofreading enzyme. What is the end structure of PCR products generated by  ExcelTaq™ Klen-Taq DNA Polymerase (TK1000)?Since ExcelTaq™ Klen-Taq DNA polymerase (TK1000) is blended KlenTaq-1 DNA polymerse and a small amount proofreading enzyme, the end structure of PCR products generated by  ExcelTaq™ Klen-Taq DNA Polymerase will be mixture of 3’-dA overhangs and blunt ends.  Recommended PCR Condition  Template1– 150 ng Forward primer0.1– 0.5 µM Reverse primer0.1– 0.5 µM 10X Klen Buffer5µl dNTPs0.2mM (each) Klen-Taq enzyme0.5µl (2.5 U) ddH2Oto50 µl Total volume50µl  Recommended PCRProgram StepsTemp.TimeCycles Template denature94°C2 min1 Denature94°C30 sec25-40 Annealing50-68°C30 sec Extension68°C30 sec/kb Final extension68°C1 min1 [RP1000] ExcelRT™ Reverse TranscriptaseHigh yieldThermostable, up to 50°C, during first strand synthesisHigh processivity, generating cDNA up to 8 kbReduced RNase H ribonuclease activity[TF1000] SMO-HiFi™ DNA Polymerase5’→3’ DNA polymerase activity3’→5’ exonuclease (proofreading) activityHigh reaction rate—10 seconds/kbHigh fidelity— 70 times higher than Taq polymeraseGenerates blunt end ampliconThermo-stable for more than 10 hrs at 95°C.[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) High sensitivity and signal intensity Compatible with fast PCR programWith smart blue contrast dye as a visual aid for reaction setup Low backgroundHigh stability[TP1200] ExcelTaq™ 5X PCR Master Dye Mix5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced  pipetting errorsIncludes tracking dye for direct loading after PCR