产品说明
Product overview Technical/Spec Support/Documents Related products Description The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis FeaturesGenerates dsDNA directly from RNA samples High sensitivity and yields High reproducibility, reduced pipetting errorsHigh throughput Reduced RNase H ribonuclease activityStorage-20°C for 24 months ContentsComponentVolumeTaq /RT Enzyme Mix50 μl2X One-Step Buffer2 x 750 μlStorage-20°C for 24 months High sensitivity and yields ManualManual_RP1100_ExcelRT™ 2X One-Step RT-PCR KitSDSSDS_RP1100[RP1000] ExcelRT™ Reverse TranscriptaseHigh yieldThermostable, up to 50°C, during first strand synthesisHigh processivity, generating cDNA up to 8 kbReduced RNase H ribonuclease activity[RI1000] RNAok™ RNase InhibitorApplicationRT-PCRcDNA Synthesis in vitro transcription[NS1000] FluoroVue™ Nucleic Acid Gel StainExcellent for in-gel stainingSensitivity up to 0.14 ng DNA or 1 ng total RNAA safer alternative to EtBr Suitable to blue or UV light [RQ2110] ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)High yieldReverse transcription at wide temperature range (42-60°C)High specificity Suitable for fast cycle programWith ROX reference dye
SMOBIO的B-BOX™是一种长波长蓝光LED落射照明器。它设计紧凑，结构坚固。B-BOX™落射照明器由于其非紫外线光源和低至12伏的低工作电压，并且为非致癌DNA /蛋白质染料提供了工作能力，因此为用户提供了前所未有的安全性。B-BOX™带有高质量的非闪烁LED灯，对DNA和蛋白质染料高度敏感。B-BOX™可以极大地减轻凝胶提取的日常琐事，即使在明亮的环境光下也可以轻松进行可视化和凝胶切割。多角度滤板（目录号VE0102）为凝胶切割，可视化和记录提供了最佳角度。B-BOX™还带有用于过滤和可视化的琥珀色滤镜（目录号VE0103）。Phox™Photobox（目录号 VE0104）为使用任何智能手机拍摄的图像提供了一个迷你暗室环境。最后，工作区域周围的内置屏障系统有助于清洁。
特征
提高克隆效率
紧凑，轻巧和便携式（重量不足1千克）
安全功能包括：470 nm长波长，对用户没有任何紫外线辐射危害
与非致癌，非溴化乙锭的DNA染料兼容
用户友好：样品易于观察（使用滤板或护目镜时）
LED光源持续长达50,000小时
出色的检测灵敏度：使用FluoroStain™DNA荧光染色染料时，DNA≤0.04 ng，使用FluoroStain™蛋白荧光染色染料时，蛋白≤3ng（与银染一样灵敏）
可调和可移动的滤板可进行凝胶切割，可视化和记录
内置屏障设计，易于清理
在明亮的环境光下可见
借助其自身的内置散热器，强调了最小的功率依赖，低发热
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