Product overview Technical/Spec Support/Documents Tips for usage Related products  DescriptionThe ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently. Features5’→3’ DNA polymerase activityNo detectable 3"→5" exonuclease (proofreading) activityGenerates PCR products with 3"-dA overhangsHigh yield PCR High reproducibilityReduced  pipetting errorsIncludes tracking dye for direct loading after PCRApplications Routine PCRColony PCRHigh throughput PCRAmplification of DNA fragments up to 8 kbGeneration of PCR products for TA cloningDNA labelingStorage4°C for 6 months-20°C for 24 monthsElongation capabilityExcelTaq™ PCR Master Mix can reliably amplify λDNA up to 8 kb in length. (M: DM3100) ContentsComponentVolumeCat. NoExcelTaq™ 5X PCR Master Dye Mix2 x 1 mlTP1200 (200 Rxn)Storage4°C for 6 months-20°C for 24 months ManualManual_TP1200_ExcelTaq™ 5X PCR Master Dye MixSDSSDS_TP1200   Recommended PCR ConditionTemplate1 – 150 ngForward primer0.1 – 0.5 µMReverse primer0.1 – 0.5 µM5X PCR Master Dye Mix10 µlH2Oto 50 µlTotal volume50 µlRecommended PCR ProgramStepsTemp.TimeCyclesTemplate denature94°C2 min1Denature94°C30 sec25-40Annealing50-68°C*30 secExtension72°C30 sec/kbFinal extension72°C1 min1*Optimal PCR condition varies according to primers’ thermodynamic properties.High Fidelity PCR amplificationAmplification of target gene with HiFi™ DNA polymerase to minimize error rate.[TF1000] SMO-HiFi™ DNA Polymerase, (1 U/μl, 100 U)[TF3000] G-HiFi™ DNA Polymerase, (1 U/μl, 100 U)Gel electrophoresisStaining amplicons with safe fluorescent dyes, following by observation under blue-light illuminator to minimize damage of DNA amplicons and maximize successful cloning efficiency.   Safe fluorescent dyes[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl[DS1000] FluoroStain™ DNA Fluorescent Staining Dye (Green, 10,000X), 500 μl[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 mlBlue-light illuminator[VE0100] B-BOX™ Blue Light LED Epi-illuminatorLigationBlund-end PCR amplicons can directly ligate with PCR cloning vector.   [CV1100] GetClone™ PCR Cloning Vector II, 20 RxnTransformationPrepare competent cells with high efficiency and transform with time-saving protocol.[CK1000] Champion™ E. coli Transformation Kit