描述Thequbit4FlurometeristhelatestversionofthepopularQubitfluorometerdesignedtoaccuratelymeasureDNA,RNA,andproteinquantity,andnowalsoRNAintegrityandquality,usingthehighlysensitiveQubitassays.TheQubit4Fluorometerwasre-engineeredtoruntheQubitRNAIQ(integrity&quality)assay.TheQubit4FluorometerandRNAIQAssayKitworktogethertoaccuratelydistinguishintactfromdegradedRNAinjusttwoeasysteps.ForallQubitassays,theconcentrationorqualityofthetargetmoleculeinthesampleisreportedbyafluorescentdyethatemitsasignalonlywhenboundtothetarget,whichminimizestheeffectsofcontaminantsontheresult.Theeasy-to-usetouch-screenmenusmakeiteasytoselectandruntheassaysyouneed,withresultsdisplayedinjustafewseconds.KeyfeaturesoftheQubit4Fluorometerinclude:•FastandhighlyaccuratequantitationofDNA,RNA,andproteininlessthanthreesecondspersample•MeasuresintactRNAinlessthan5secondspersample•Highlevelsofaccuracyusingonly1–20μLofsample,evenwithverydilutesamples•On-boardReagentCalculatorquicklygeneratesQubitworkingsolutionpreparationinstructions•Storesresultsfromupto1000samples•Large5.7-inch,state-of-the-artcolortouchscreenforeasyworkflownavigation•Graphicaldisplayindicateswhensamplesareintheextendedrangeoroutofrange•Smallfootprintsavesspaceonyourbench•ExportsdatatoaUSBdriveordirectlytoyourcomputerviaaUSBcable•ABIlitytopersonalizeyourQubitfluorometerwiththeassaysyourunmost,addnewassays,orevencreateyourownassayswiththeMyQubitsoftwareandwebtool•LanguageofyourchoiceincludingEnglish,French,Spanish,Italian,German,simplifiedChinese,andJapaneseTheQubit4FlurometercanalsobeusedtodirectlymeasurethefluorescenceofsamplesusingFluorometermode.Additionally,IonSphereParticlequalitycanbeevaluatedontheQubit4FluorometerusingtheIonSphereQualityControlKitpriortoperformingasequencingrunontheIonPGMsequencer.EnablesmoreaccurateresultsthanwithUVabsorbanceatlowconcentrationsTheQubit4FluorometerisfarmoresensitivethanUVabsorbance,whichmeasuresanythingabsorbingat260nm,includingDNA,RNA,protein,freenucleotides,orexcesssalts.Moreover,UVspectrophotometryisoftennotsensitiveenoughtoaccuratelymeasurelowconcentrationsofDNAandRNA.WiththeQubitfluorometer,yourresearchisenhancedbymoreaccuratemeasurementsbecausethedyesintheQubitassaykitsfluoresceonlywhenboundtotheselectedmolecule(DNA,RNAorprotein)inyoursample.Thisallowsyoutoavoidrepeatingworkduetoinaccuratemeasurements.Fast,simple,andaccuratemeasurementofRNAqualityTheQubitRNAIQassayprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegraded.Theassayutilizestwouniquedyes:onethatbindstolarge,intactRNA,andanotherthatselectivelybindstosmall,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample.Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.SavesamplesandbenchspaceThethoughtfullydesignedQubit4Fluorometerrequiressamplesofonly1–20µL.Itisintendedforuseatroomtemperatureandoccupiesonlyasmallamountofbenchspace.TheQubit4Fluorometerfeaturesadvancedopticsanddataanalysisalgorithms,andcomesequippedwithaUSBthumbdriveandcablefordatatransfertoExcel™softwareandforsoftwaredownloads,aswellasauniversalpowersupply,fourplugadapters,andelectromagneticCEcertification.SinglesamplemeasurementsareabreezeThisuser-friendlyfluorometeriscombinedwithquickandsimpleassayprocedures.Calculationsandsettingsareautomaticallyperformedbytheinstrument.TheQubitassaysforusewiththeQubit4Fluorometerareallperformedusingthesamegeneralprotocol,whichusesasimplemix-and-readformatwithincubationtimesofonlytwominutesfortheDNAandRNAassays.500Lthin-walledPCRtubes(Cat.No.Q32856)arerequired.Systemspecificationsinclude:Instrumentdimensions: 5.4"(w)x10"(l)x2.2"(h)(13.6cmx25cmx5.5cm)Weight: 743gDynamicrange: 5ordersofmagnitudeProcessingtime: ≤seconds/sampleLightsources: blueLED(max~470nm),redLED(max~635nm)Excitationfilters: blue(430–495nm),red(600–645nm)Emissionfilters: green(510–580nm),red(665–720nm)Detectors: photodiodes,measurementcapabilityfrom300–1,000nmWarm-uptime: <35secondsUSBthumbdrive: 4GB