Human Alpha 1-Antichymotrypsin ELISA KitCATALOG # E-80CYTHuman Alpha 1-Antichymotrypsin (SERPINA3) ELISA Kit.Product Citations:Wågsäter D, et al.Serine protease inhibitor A3 in atherosclerosis and aneurysm diseaseINTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 30: 288-294, 2012Manal L NIAZI, M.D. and MAGED ABD EL-NASEER, M.DSerum Inflammatory Markers in Dementia Disorders Med. J. Cairo Univ., Vol. 77, No. 4, June: 337-346, 2009 Size1.0 platePrice$315.00QuantityAdd to CartORRequest Bulk QuoteAdd to WishlistDetailsReactivityHumanHostGoatAntibody typeELISASpecificity/TargetAlpha 1-AntichymotrypsinStorage conditions2-8CSample TypePlasma, SerumRange6.25 ng/ml - 400 ng/mlSensitivity Average2.518 ng/mlTest Time80 min.Principle Of The AssayThe principle of the double antibody sandwich Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA is represented in Figure 1. In this assay the Alpha 1-Antichymotrypsin present in samples reacts with the anti-Human Alpha 1-Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1-Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1-Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1-Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1-Antichymotrypsin in the test sample. The quantity of Alpha 1-Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. DocumentsData SheetSDSKit Contents1One ELISA Micro Plate with 12 removable (8 well) micro well strips in holding frame, each coated with Affinity Purified Antibody2One ELISA Kit Data Sheet3One Certificate of Analysis4One 50 mL bottle of Diluent Running Buffer5One 50 mL bottle of 20X Concentrated Wash Solution6One 150 uL Vial of Affinity Purified HRP Conjugated Antibody in stabilizing buffer7One 12 mL vial of Chromogen-Substrate Solution8One 12 mL vial of Stop Solution9One Calibrator VialCitationsSubmit Your Citation $150 COUPON Publishing research using our antibodies or ELISA kits? TELL US AND RECEIVE A $150 COUPONThis product is for research use only, not for diagnostic or therapeutic use.