首页 > Itsi-Biosciences > Itsi-Biosciences/Protein Isolation Kit for Two-Dimensional Difference Gel Electrophoresis [ToPI-DIGE; Cat No.: K-0010]/10 Assays/K-0010-C10

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Itsi-Biosciences/Protein Isolation Kit for Two-Dimensional Difference Gel Electrophoresis [ToPI-DIGE; Cat No.: K-0010]/10 Assays/K-0010-C10

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K-0010-C10

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Itsi-Biosciences

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The ToPI-DIGE™ Kit contains a set of optimized reagents and standard operating procedures that allow basic and clinical research scientists to easily, conveniently and reproducibly isolate total proteins from human and non-human cell lines (attached and suspended), solid tissue and tumor biopsies for use in 2D-DIGE experiments (Fig 1).ToPI-DIGE is optimized for isolation of total proteins from cell lines (K-0010-C) and mammalian tissue (K-0010-T) for 2-Dimensional difference in gel-electrophoresis (2D-DIGE). Using the kit prevents protein breakdown and prevents additional buffer exchange steps that may lead to protein breakdown and lower yield.(Protease-, RNase- & DNAse- free) Order NowFig 1: 2D-DIGE image of Human Breast Cancer Cell Lines Proteins were isolated with ToPI-DIGE Kit. 50ug of protein from “reference”, “test” and “Internal Standards” were labeled with Cyanine dyes and loaded on the gel.Each Kit contains optimized buffer systems and consumables including:Buffer 1Buffer 2Buffer 3iTube AiTube B (Protease-, RNase- & DNAse- free)Validated SOP for total protein isolation from cell lines and tissue for 2D-DIGEK-0010-C10 (10 Assays) – $68.00K-0010-T10 (10 Assays) – $65.00 Click Here to Order on Amazon

ITSI-Biosciences LLC由具有广泛学术和行业经验的资深科学家于2004年成立，提供生物科学DNA测试和蛋白质测试服务，以支持法医学，生物医学研究，蛋白质组学和基因组学。我们支持政府机构，例如司法部，食品和药物管理局，国立卫生研究院，美国农业部，能源部和国防部以及制药公司和大学。

凭借40多年的综合经验和可用的最新技术，我们可以从最困难的人类和非人类标本中提取DNA和蛋白质，并以目前可能的最全面方式分析DNA和蛋白质。通过提供DNA和蛋白质水平的测试，我们的法医学部门可以对组织样本或生物污渍进行个性化处理，并明确识别污渍的来源。