应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 抑制或激活实验, 其他更多信息或购买: Enzo Life Sciences 产品网页出版应用/物种/样品/稀释参考文献流式细胞仪; 人类; 图1aBreiman A, L????pez Robles M, de Carn???? Tr????cesson S, Echasserieau K, Bernardeau K, Drickamer K, et al. Carcinoma-associated fucosylated antigens are markers of the epithelial state and can contribute to cell adhesion through CLEC17A (Prolectin). Oncotarget. 2016;7:14064-82 pubmed出版商 其他; scFv; 2 ug/ml; 图4Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, et al. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells. J Biol Chem. 2015;290:20071-85 pubmed出版商 其他; scFv; 图3b酶联免疫吸附测定; 人类; 图2aZhang D, Huang P, Zou L, Lowary T, Tan M, Jiang X. Tulane virus recognizes the A type 3 and B histo-blood group antigens. J Virol. 2015;89:1419-27 pubmed出版商 抑制或激活实验; Trachemys dorbigniBenktander J, Angstrom J, Breimer M, Teneberg S. Redefinition of the carbohydrate binding specificity of Helicobacter pylori BabA adhesin. J Biol Chem. 2012;287:31712-24 pubmed出版商 流式细胞仪; 人类Suila H, Pitk??nen V, Hirvonen T, Heiskanen A, Anderson H, Laitinen A, et al. Are globoseries glycosphingolipids SSEA-3 and -4 markers for stem cells derived from human umbilical cord blood?. J Mol Cell Biol. 2011;3:99-107 pubmed出版商 Nakao H, Matsumoto S, Nagai Y, Kojima A, Toyoda H, Hashii N, et al. Characterization of glycoproteins expressing the blood group H type 1 epitope on human induced pluripotent stem (hiPS) cells. Glycoconj J. 2017;34:779-787 pubmed出版商 Li D, Breiman A, Le Pendu J, Uyttendaele M. Binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress. Front Microbiol. 2015;6:659 pubmed出版商 Nystr????m K, Abrantes J, Lopes A, Le Moullac Vaidye B, Marchandeau S, Rocher J, et al. Neofunctionalization of the Sec1 ??±1,2fucosyltransferase paralogue in leporids contributes to glycan polymorphism and resistance to rabbit hemorrhagic disease virus. PLoS Pathog. 2015;11:e1004759 pubmed出版商 Eller C, Yang G, Ouerfelli O, Raines R. Affinity of monoclonal antibodies for Globo-series glycans. Carbohydr Res. 2014;397:1-6 pubmed出版商 Huang C, Thayer D, Chang A, Best M, Hoffmann J, Head S, et al. Carbohydrate microarray for profiling the antibodies interacting with Globo H tumor antigen. Proc Natl Acad Sci U S A. 2006;103:15-20 pubmed Adobati E, Zacchetti A, Perico M, Cremonesi F, Rasi G, Vallebona P, et al. Expression profile of saccharide epitope CaMBr1 in normal and neoplastic tissue from dogs, cats, and rats: implication for the development of human-derived cancer vaccines. Histochem J. 1999;31:729-37 pubmed Cordel S, Goupille C, Hallouin F, Meflah K, Le Pendu J. Role for alpha1,2-fucosyltransferase and histo-blood group antigen H type 2 in resistance of rat colon carcinoma cells to 5-fluorouracil. Int J Cancer. 2000;85:142-8 pubmed Adobati E, Panza L, Russo G, Colnaghi M, Canevari S. In vitro mimicry of CaMBr1 tumor-associated antigen by synthetic oligosaccharides. Glycobiology. 1997;7:173-8 pubmed Perrone F, Menard S, Canevari S, Calabrese M, Boracchi P, Bufalino R, et al. Prognostic significance of the CaMBr1 antigen on breast carcinoma: relevance of the type of recognised glycoconjugate. Eur J Cancer. 1993;29A:2113-7 pubmed Martignone S, Menard S, Bedini A, Paccagnella A, Fasolato S, Veggian R, et al. Study of the expression and function of the tumour-associated antigen CaMBr1 in small cell lung carcinomas. Eur J Cancer. 1993;29A:2020-5 pubmed Mariani Costantini R, Barbanti P, Colnaghi M, Menard S, Clemente C, Rilke F. Reactivity of a monoclonal antibody with tissues and tumors from the human breast. Immunohistochemical localization of a new antigen and clinicopathologic correlations. Am J Pathol. 1984;115:47-56 pubmed Bremer E, Levery S, Sonnino S, Ghidoni R, Canevari S, Kannagi R, et al. Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland. J Biol Chem. 1984;259:14773-7 pubmed Mariani Costantini R, Colnaghi M, Leoni F, Menard S, Cerasoli S, Rilke F. Immunohistochemical reactivity of a monoclonal antibody prepared against human breast carcinoma. Virchows Arch A Pathol Anat Histopathol. 1984;402:389-404 pubmed Pubmed号列表: 25875017, 16373501, 10646838, 10585597, 9134423, 8297650, 7506562, 6711680, 6501317, 6426148克隆: 抗体亚型: 产品描述: The murine monoclonal antibody to globo H (clone MBr1) is raised against the breast cancer cell line MCF7 and recognizes a saccharidic epitope (CaMBr1) overexpressed on a high percentage of human breast, ovary lung and prostate carcinomas. This antigen was originally identified on the immunogen as a globo-series glycosphingolipid with a H-like determinant at its terminus (globo-H), identical to GL6 isolated from human teratocarcinoma. MAb to MBr1 was also found to be reactive with different glycoconjugates (glycoproteins, mucins and glycolipids) in breast and small cell lung carcinoma. Retrospective as well as prospective studies suggest that CaMBr1 expression is associated with tumor aggressiveness and survival outcome in breast and small cell lung cancer and that poor prognosis is more frequently associated with CaMBr1 expression on glycoproteins. The epitope recognized by the MAb to MBr1 consists of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal, available as a synthetic oligosaccharide exploitable for the development of new anti-cancer vaccines. Carbohydrate antigens expressed at the cell surface of human cancer cells hold promise as targets for active immunization with vaccines.CaMBR1 is also expressed on some normal tissues, and its expression restricted tissue distribution makes it a suitable target for oncologic clinical applications. In particular the MAb to MBr1 can be succesfully used for immunocytochemical detection of micrometastatic cells.物种活性: 人类, 大鼠, 狗, 猫免疫原: Membrane preparation from the human breast carcinoma cell line MCF7.特异性: Recognizes membrane bound glycoconjugates (glycoproteins, mucins and glycolipids) expressing the globo H or its tetrasaccharide terminus. Recognizes an epitope consisting of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal.缓冲液: Liquid. In 50mM PBS, pH 8.0, containing 150mM sodium chloride. Contains no sodium azide.用途: Not recommended for immunoprecipitation.应用摘要: FC, 免疫细胞化学, 免疫组化(FS), 免疫组化(PS), 免疫印迹储存: 更多信息或购买: Enzo Life Sciences 产品网页techserv-usa@enzolifesciences.comwww.enzolifesciences.com1-800-942-0430公司总部: 美国自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。