Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) Solution is an extraordinarily powerful, odorless, thiol-free reducing agent with broad application to protein and nucleic acid involving the reduction of disulfide bonds. The pH value of the solution is designed at 6.0, 7.0, and 8.0 for multiple choices. This product is an effective and convenient replacement for βmercaptoethanol (β-ME) or DTT in protein analysis.Kit ContentsThe product is supplied as a 0.5M solution at pH 6.0, 7.0, and 8.0.Directions for UseTCEP breaks disulfide bonds and can be used for a wide variety of protein applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. Its ability to prevent oxidation of protein samples makes it a useful buffer component because it helps to preserve enzymatic activity.Key FeaturesReplacement of DTT and β-ME: unlike DTT and βME, TCEP is odor-free, contributing to a healthier lab environment.High Efficient: 5 to 50mM TCEP thoroughly reduces most peptide or protein disulfide bonds within a few minutes.Stable and versatile: reduces peptides and proteins over a broad range of pH, salt, detergent, and temperature conditions. It is resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins.Compatible: removal of this reagent is not necessary before most applications, (e.g. histidine-tagged protein purification, maleimide conjugations), because TCEP does not contain sulfhydryl groups.Storage ConditionsThe product, as supplied, is stable for at least two years at 2-8℃ and shipped ambient.Related ProductsUrea Solution (8M) (TBS5037)Ammonium Sulfate Solution (4M) (TBS5038)DTT Solution (1M) (TBS5039)MOPS Buffer (1M) (TBS5041)Bicine buffer (1M) (TBS5042)Additional informationSIZE (ml)1 ML, 10 ML, 50 MLpHpH=6.0, pH=7.0, pH=8.0Datasheet:TBS5082_ TECEP_V03_2021MSDS:TBS5082_TCEPSolution Safety Be the first to review “0.5M TCEP-HCl Solution (pH 6.0, 7.0 and 8.0)”
TribioScience 是一家私人控股的生物技术公司,于 2010 年创立。我们的使命是让科学家在实验室的工作更轻松、更省时。我们专注于为分子生物学、生物化学、免疫学、传染病、基因治疗、神经科学、动物实验和药物开发领域的生命科学研究实验室和生物技术公司提供高质量的产品。我们的团队成员拥有超过 50 年的综合经验,我们还作为 CRO(研究合同组织)为生物技术行业和学术界提供服务。我们的动物建模服务已被用于 高排名大学的动物模型开发和体内评估。
TribioScience is a privately held biotech founded in 2010 by a group of scientists at Stanford University. Our mission is to make scientists’ jobs in the laboratory easier and less time-consuming. We focus on offering high-quality products for life science research laboratories and biotech companies in the areas of molecular biology, biochemistry, immunology, infectious disease, gene therapy, neuroscience, animal experiment, and drug development.