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intactgenomics/Sau DNA Polymerase Large Fragment - Intact Genomics/1000 units (5 units/µl)/3595
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产品说明
Sau DNA Polymerase I, Large Fragment is a product of the Staphylococcus aureus DNA polymerase I which lacks the N-terminal exonuclease domain (1-293 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.ApplicationsStrand displacement DNA synthesis (1)Random primer labelingSecond strand cDNA synthesisdA-tailingProtein PurityThe physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).Product SourceE. coli strain expressing Sau Polymerase I gene lacking the N-terminal exonuclease domain.Product Includes• Sau DNA Polymerase I, Large fragment• 10x Sau DNA Polymerase I reaction buffer1x Sau DNA Polymerase I reaction buffer10 mM Tris-HCl50 mM KCl10 mM MgCl21 mM DTTpH 7.9 @ 25°CStorage Buffer50 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA,50% GlycerolpH 7.5 @ 25ºCStorage Temperature–20ºCHeat inactivation70°C for 20 minUnit DefinitionOne unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C.Quality Control assaysSau Polymerase I, Large fragment is free from detectable nuclease activities. Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.References1. Piepenburg, O. et al. (2006) PLOS Biology, 4, 1115–1121.3592 3595
Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备,位于西线工业园,靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。
我们提供高质量的生命科学产品,包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务,以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。
我们还在宏基因组研究中应用BAC相关技术,为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司,我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。
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