Intact Genomics (IG) i7® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace (1). It is ideal for cloning and can be used for long (up to 20kb) or difficult amplicons. This product is supplied with the Intact Genomics proprietary 2.5x i7® PCR reaction buffer containing MgCl2 with a final (1x) concentration of 2 mM. This buffer allows for amplification of non GC rich templates and of GC rich templates up to 84%.Protein PurityThe physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).Product SourceE. coli strain expressing genetically engineered i7® High-Fidelity DNA Polymerase gene.Comparison DataWe have tested this DNA Polymerase activity with λ DNA and other difficult templates for PCR amplification up to 20kb. Intact Genomics (IG) i7® high-fidelity DNA Polymerase generates robust and high-quality PCR products in comparison with other high-fidelity DNA polymerases available in the marketplace (data shown below):ApplicationsLong and difficult template DNA amplificationCloningHigh-fidelity PCREfficient for amplifying high GC content template DNA with magic enhancerProduct Includesi7® High-Fidelity DNA Polymerase2.5x i7® PCR Buffer with Mg2+Storage Buffer50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC Storage Temperature–20ºCHeat InactivationNoQuality Control AssaysFree from detectable nuclease activities.Unit DefinitionOne unit is defined as the amount of enzyme thatincorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72º C.3254 3255