首页 > intactgenomics > intactgenomics/Glycerol Free T4 gp32 Protein | ig | Intact Genomics/500µg (10µg/µl)/3515G

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intactgenomics/Glycerol Free T4 gp32 Protein | ig | Intact Genomics/500µg (10µg/µl)/3515G

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T4 gp32 Protein is a single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair (1, 2). It improves the efficiency of reverse transcriptase (RT) during RT-PCR (3), enhances T4 DNA polymerase activity (4), as well as increases the yield of PCR products (5).Note:Glycerol acts as a cryoprotectant and protein stabilizer when added to the storage buffer of proteins and enzymes. However, in certain circumstances, it is preferred to omit glycerol from the buffer. This includes instances where the presence of glycerol may interfere, such as lyophilization, high-throughput instruments with sensitive fluidics or primary cell cultures.For our glycerol free products, it is recommended to use immediately upon thaw as without glycerol there will be significant activity loss with freeze/thaw cycles.Protein PurityThe physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining (Fig. 1).Product Source coli BL21 (DE3) strain expressing T4 gp32 gene.Product IncludesGlycerol Free T4 gp32 protein10X gp32 reaction buffer1x GP 32 reaction buffer composition20 mM Tris-acetate100 mM Potassium acetate10 mM Magnesium acetate1 mM DTTpH 7.8 @ 25ºCStorage Buffer50 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTApH 7.5 @ 25ºCStorage Temperature–20ºCHeat Inactivation65°C for 20 min Quality Control assaysT4 gp32 protein is free from detectable nuclease activities.ReferencesChase, J. W. and Williams, K. R. (1986) Ann. Rev. Biochem. 55, 103-136Sinha, N. K. and Snustad, D. P. (1971) J. Mol. Biol. 62, 267-271.Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.Topal, M.D. and Sinha, N.K. (1983). J. Biol. Chem. 258, 12274-12279.Schwartz, K. et al. (1990). Nucl. Acids Res. 18, 1079.3512 3515

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

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