Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Description
Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
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Equipment | Microcentrifuge, vortex, magstand |
Sample Inactivation | TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
Size Range | Total RNA ≥ 17 nt |
Yield | 10 µg RNA (binding capacity), ≥ 30 µl (elution volume) |
Q1: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q2: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q3: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q4: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME!It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
-A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
-Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
-Arjan V. (Indiana University)
Read MoreCat # | Name | Size | Price | |
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D4100-2-3 | MagBinding Beads | 3 ml | $66.00 | |
D4100-2-12 | MagBinding Beads | 12 ml | $125.00 | |
W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 | |
R1060-2-100 | RNA Prep Buffer | 100 ml | $122.00 | |
R1060-2-25 | RNA Prep Buffer | 25 ml | $40.00 | |
R2100-1-20 | Direct-zol Binding Buffer Concentrate | 20 ml | $84.00 | |
R2100-2-200 | Direct-zol MagBead PreWash | 200 ml | $174.00 | |
R2130-1-120 | MagBead DNA/RNA Wash 1 | 120 ml | $198.00 | |
R2130-1-30 | MagBead DNA/RNA Wash 1 | 30 ml | $63.00 | |
R2130-2-20 | MagBead DNA/RNA Wash 2 | 20 ml | $54.00 | |
R2130-2-80 | MagBead DNA/RNA Wash 2 | 80 ml | $171.00 | |
C2002 | Collection Plate | 2 Plates | $22.00 | |
C2003 | Elution Plate | 2 Plates | $19.00 | |
C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
E1010 | DNase I Set | 250 U | $56.00 |
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解析:1.细胞质基质(也叫胞质溶胶)是指除细胞器外细胞质的其余部分。细胞质基质是活细胞进行新陈代谢的主要场所。
2.细胞新陈代谢的次要场所是:细胞核、线粒体基质、叶绿体基质等基质。
2015年7月6日讯/生物谷BIOON/--最近,来自艾默里大学的科学家发现在许多黑色素瘤中存在一个重要基因突变能够使癌细胞的代谢重新连线,使癌细胞的生长依赖于一种参与酮体生成的催化酶,这一发现为解决黑色素瘤细胞对靶向药物的抵抗,开发新的替代药物提供了深入见解,同时也部分解释了为什么这一突变在黑色素瘤细胞中频发。近日,相关研究结果发表在国际学术期刊molecularcell。
B-raf基因发生V600E突变在黑色素瘤细胞中非常常见,这一突变能够促进癌细胞生长,除了在黑色素瘤中存在,在一些结肠癌和甲状腺癌病例中也发现存在B-rafV600E突变。目前已经开发出一些针对B-rafV600E基因突变的靶向药物,但在临床实验中发现,在癌症得到明显改善之后,携带V600E基因突变的癌细胞都不可避免地产生药物抗性。
在这项研究中,研究人员发现B-rafV600E基因突变能够刺激癌细胞产生更多的HMG-CoA裂解酶,携带V600E突变的黑色素瘤细胞生长非常依赖于该酶,而其他的黑色素瘤细胞则不会。HMG-CoA裂解酶是酮体生成途径中一个重要酶,能够帮助机体在血糖水平较低时降解脂肪酸以获得能量。酮体生成途径能够受到低糖,高脂饮食刺激激活,通常发生于肝脏,但B-rafV600E基因突变启动了癌细胞中的酮体生成,以维持癌细胞生长存活。除此之外,研究人员还发现酮体生成途径的重要产物乙酰乙酸能够刺激携带B-rafV600E基因突变的癌细胞继续增殖。
总得来说,这项研究证明B-rafV600E基因突变能够使黑色素瘤细胞中的代谢途径重新连线,增强癌细胞对酮体生成途径的依赖性,这一发现对于解决黑色素瘤细胞对靶向药物的抵抗,开发新的替代药物具有重要意义。(生物谷Bioon.com)
步骤/方法
将双手放于下巴处,用手指的指腹分别以转圈的方式向耳部方向按摩,并在耳部下方轻轻按压。反复按摩并持续2分钟。由于淋巴是毒素排出的重要渠道,反复按摩此处.有助于琳巴疏通,使毒素顺利排出
双手从下巴处以画圈的方式向嘴角按摩,并在嘴角处轻轻按揉,反复按摩并持续2分钟。下巴常常会堆积很多的毒素,也是比较容易生出粉刺和痘痘的地方。经常对下巴进行按摩可以有助于毒素的排出
.双手无名指指腹放在眼角部位沿着鼻子两侧轻轻从上住下按摩,反复按摩并持续2分钟,此动作可以促进鼻子子部位的血液循环,有助于顺利排出毒素
手指并拢,将双手指腹放在眼睛下方,慢慢向两耳处轻轻按摩,然后再从鼻子中间两侧的脸颊处向耳朵方向轻轻按摩,反复按摩3分钟。此按摩可以加速脸部的血液循环,促进脸部细胞的活化,有助于排除毒素
将双手分别放于嘴角处,然后以画圈的方式向着耳朵的方向轻轻按揉。由于嘴角到耳朵的沿线分布普很多的穴位,经常按摩可以促进细胞的新陈代谢,排出毒素,还可以减缓衰老
用液氮淬灭是怎么淬灭法?
具体应该怎么操作?
请不吝赐教!!谢谢各位大大!!
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