Highlights
- Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
- Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
- Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.
Description
Applicable For | High-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications |
---|---|
Elution Volume | ≥ 10 µl |
Equipment | Microcentrifuge |
Purity | High-quality, purified DNA is especially well-suited for sequencing and ligation reactions |
Sample Source | Agarose gels containing large DNA fragments of interest |
Sample storage | Eluted DNA can be used immediately or stored at ≤ -20°C |
Size Range | 50 bp to 200 kb |
Type | Large DNA |
Yield | Up to 10 µg DNA |
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”
-Kelly-Anne F.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
Read MoreCat # | Name | Size | Price | |
---|---|---|---|---|
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
C1002-50 | Zymo-Spin IC-XL | 50 Pack | $66.00 | |
C1002-25 | Zymo-Spin IC-XL | 25 Pack | $34.00 |
ebiomall.com
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一抗的英语是 resistance No.1,二抗resistance No.2.
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。
暂无品牌问答