8eggsperday,day7-day13 cutCAM,washinprecooledPBS, in10mlWASH1(PBS,5mMEDTA,COMPLETE)onice cutinpiecesinpetridishonice centrifuge4minat1000rpm(tabletopcentifuge,Falcon15mltubes)at4°C(precoolHeraeuscentrifugeinE2) aspiratesupernatant,add10mlWASH2(PBS,5mMEDTA,5µg/mlLeupeptin+0.5mMPefablocSC) centrifuge4minat1000rpm(tabletopcentifuge,falcon15mltubes)at4°C aspiratesupernatant,add5mlWASH2 centrifuge4minat1000rpm(tabletopcentifuge,falcon15mltubes)at4°C aspiratesupernatant,add1-1.5mlWASH2,transfertopreweighed2mlEppendorftubes,optionalrinseFalcontubewithca.0.5mlWASH2 centrifuge5minat2000rpm(Eppendorfcentrifuge)at4°C(precoolEppendorfcentrifugeinE7?) aspiratesupernatant,weighandstoreat-20°C -COMPLETEBoehringer169749820tablets345CHF -LeupeptinSigmaL-064925mg185,90CHF -PefablocSC(AEBSF,4-(2-Aminoethyl)-benzenesulfonylfluoride,hydrochloride)Boehringer1429868100mg75CHF