IsolationofRNAfromwholewormsusingTRIZOLreagent(Gibco-BRL). 1)Washwormsfroma60mmplatewith1mlDEPC-treatedwater. 2)Spindownwormsat4000xginmicrofugefor1minute. 3)Removeexcesswaterandadd1mlTrizolreagent.Vortexandinverttube.Letsitatroomtermeraturefor10minutes(shouldseestringy-likematerial). 4)Spintubesinmicrofugeat14Kfor10minutesat4°C. 5)RemovesupernatantintofreshRNAsefreeEppendorftubeandadd200µlofchloroform. 6)Vortexfor15secondsandletsitatroomtemperaturefor3minutes. 7)Spininmicrofugeat12Kfor15minutesat4°C. 8)Carefullyremovethetoplayer(clear)intoanewRNAsefreeeppendorftubeandadd500µlofIsopropanol.Inverttomix.Letsitfor10minutesatroomtemperature. 9)Spininmicrofugeat12Kfor10minutesat4°C.(RNAwillbeseenasanicewhitepellet). 10)Washpelletwith100µlof75%ethanol(madebydilutingintoDEPC-treatedwater). 11)Spininmicrofugeat7500xgfor5minutesat4°C. 12)Removesupernatantandairdrypelletfor10minutes. 13)Dissolvepelletin25µlofDEPC-treatedwater.Heatfor10minutesat60°CtohelpdissolveRNA.
