MycoplasmatestsaccordingtotheEuropeanPharmacopoeia(EP)Chapter2.6.7requireasensitivityof10colonyformingunits(CFU)permlsamplevolumeforNAT-basedmethodssuchasPCRinordertoreplacetrADItionalculturemethods.Thissensitivitymustbeaccomplishedaspartoftherobustnesstestingforanyparticularsamplematrix.
Usingvitalmycoplasmaisnotacceptableforthemajorityofcellculturelaboratoriesduetosafetyregulations.Our10CFU™sensitivitystandardscontainnon-vitalmaterialandallowsafeandreliablevalidation.
ThemycoplasmaweregrowninculturemediumasdescribedinEP2.6.7,subsequentlytitratedandplatedonHayflickandFreymediumforCFUdetermination.Themycoplasmawereharvestedintheearlylogarithmicgrowthphasetoensureahighratioofvitaltonon-vitalmycoplasmaandthusalowratioofGU*toCFU.AllstrainswereobtainedfromtheNationalCollectionofTypeCultures(NCTC),UK,andcultivatedinlowpassages.
ScopeofApplication
10CFU™SensitivityStandardsareintendedforvalidatingtherobustnessandsensitivityofNAT-basedmycoplasmatests.
KitComponents
Unitpackage:3vialscontaining10cfuoftheparticularmycoplasmaaswellas3vialsnegativecontrol
Setpackage:2vialsofeachEP2.6.7-listedmycoplasmacontaining10cfu(18vialsintotal)aswellas2vialsnegativecontrol
Thecomponentsarelyophilized.
SpeciesandOrderInformation
- Cat.No.102-1003Mycoplasmaarginini
- Cat.No.102-2003Mycoplasmaorale
- Cat.No.102-3003Mycoplasmagallisepticum
- Cat.No. 102-4003Mycoplasmapneumoniae
- Cat.No.102-5003Mycoplasmasynoviae
- Cat.No.102-6003Mycoplasmafermentans
- Cat.No. 102-7003Mycoplasmahyorhinis
- Cat.No.102-8003Acholeplasmalaidlawii
- Cat.No. 102-9003Spiroplasmacitri
- Cat.No. 102-0002MycoplasmaSet
Evaluation
ApplicationofaPCR-basedmycoplasmatest,e.g.Venor®GeMqEPMycoplasmaDetectionkit
RequiredConsumables
Sterile1.5mlreactiontubes,filtertips,appropriateDNAextractionkit
Requiredlabdevices
Microcentrifuge,microPipettes
ShelfLifeandStorage
Thedateofexpiryisgivenoneachpackage.Theunopenedkitshouldbestoredat+2to+8°C.
Figure–10CFUwerere-sUSPendedin1mlcellculturemedium,200µlwereusedforDNAextractionwiththeVenor®GeMSamplePreparationKit.ThemycoplasmaPCR-testwasperformedwiththeMicrosart®ATMPMycoplasmakitandaCorbettRotorGene®6000cycler.
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做western时,看着膜上的Marker条带是直的,但是最后显影出来的目的蛋白的条带全部是歪的,这是为什么?两块胶都是这个问题,欲哭无泪呀!!!求高人指点一下,是什么操作不当导致的
想看下人肿瘤组织内巨噬细胞的浸润情况,我以为一个CD11b就够了。咨询了一下抗体公司,竟然让我订三个抗体,分别是CD11b,CD68和CD163,后两者不是M1和M2的分型吗?
另外查了下CD11b也确实在别的一些免疫细胞有所表达,请教下,有专门的特异性抗体吗?没课题没经费,只能省省省了
已经重复做了4次了,还是曝出9条带。请求各位大神解析解析==
连续三次都这样,Marker的泳道的位置有很多亮条带,但Marker本来的条带是不亮的。我做了内参和目的,一张胶上左右两部分分别都加样品和Marker,切开转膜分别孵育,一块儿显影,内参的Marker就不会亮,只有目的那一张Marker会亮,昨天做的借了别人的Marker,显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮,Marker的部分非常亮。
英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识,标记; 记号笔,阅卷人; 防守队员; 特征复数: markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
请教大家一个问题,不同厂家cIEF的Marker能混合使用吗?另外,如果我对其它厂家pI值为6.0的marker进行cIEF测定,那测得的pI值会等于6.0吗?谢谢!
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