3DCellUSPongeismadeofhydroxypropylcellulose,aknownbiocompatIBLematerial,asthebasicmaterialthatisside-chainmodifiedforcrosslinkingandligandconjugation.
  |  |  |
Thespongedisksarepre-sterilizedandreadyforcellseeding.Thecellseedingiseasy.Itcouldbeperformedthroughabsorptionofcellsuspensionwithdesiredcelldensityontothesurfaceofthedrysponge.After30-60minutesofincubation,freshpre-warmedculturemediumcouldbeaddedforextendedcellculturemaintenance.
TheCelluspongesareavailablein3forms:original,galactosylated,conjugatedwithcollagen.Manyresearchershaveusedtheseproducts,andtheyhavesuccessfullydemonstratedspheroidformationusingmanycelltypes.
-Celluspongeisbestforcellsrequiringnospecificligandandvariouscancercelllines(i.e.MCF-7&MDA-MB,NIH3T3,humanforeskinfibroblastHFF);
-Cellusponge-Galisbestforhepatocytesusedininvitrodrugtestinganddrugdevelopment(i.e.primaryrathepatocytes,primaryhumanhepatocytes,hepatocytecelllines);
-Cellusponge-Collisbestforcellsrequiringcollagensubstratum(i.e.mouseandhumanembryonicstemcellsmESC&hESC,humanmesenchymalstemcells,primarycells).
Features:
1.Hydrogel-basedmacroporoussponge(exclusivepatentlicense).
2.Simpleandefficientcellseedingprocedure.
3.Cellsdistributedwellinthespongeporesandcouldgrowwell.
4.Formfunctionalspheroidswithin24hourspostseedingandremianstableforatleast40days.
5.Appropriatespheroidsizethatpreventsapoptosisinthespheroidscenter.
6.Compatiblewithwelledplatesandinserts.
Advantages:
1.Naturalsource
2.Spheroidsizeiscontrollableandunder200um.
3.Seedingisthesameasin2Dcellcultureanddoesnotrequireextrasteps.
4.Cellsgrowrapidlywithproperfunctions.
5.Compatibletoautomatedequipment.
Applications:
1.Generalsofttissue3Dculture
2.Spongeforinvivostudy
3.Stemcelldifferentiationplatforms
4.3Dprimarycellculturemodel
5.Drug/anti-viraltesting
6.Suitableforcryopreservation
ProductFormandPackageInformation:
Cellusponge | Product(Thickness:1mm) | Catalog# |
9-mm/24disks | Cellusponge | S92424 |
(24-wellplate) | Cellusponge-Galactose | G92424 |
| Cellusponge-Collagen | C92424 |
6-mm/48disks | Cellusponge | S64848 |
(48-wellplate) | Cellusponge-Galactose | G64848 |
| Cellusponge-Collagen | C64848 |
9-mm/8disks | Cellusponge-Galactose | G92408 |
(24-wellplate) | Cellusponge-Collagen | C92408 |
6-mm/16disks | Cellusponge-Galactose | G64816 |
(48-wellplate) | Cellusponge-Collagen | C64816 |
3DCultureReferences:
1.Preparationofthree-dimensionalinterconnectedmacroporouscellulosichydrogelsforsofttissueengineering.
2.Arobusthigh-throughputsandwichcell-baseddrugscreeningplatform.
3.Purpose-drivenBiomaterialsresearchinliver-tissueengineering.
4.Galactosylatedcellulosicspongeformulti-welldrugsafetytesting.
5.Tetheredspheroidsasaninvitrohepatocytemodelfordrugsafetyscreening.
6.Morphologyandorganizationoftissuecellsin3Dmicroenvironmentofmonodispersefoamscaffolds.
7.Electrotaxisoflungcancercellsinorderedthree-dimensionalscaffolds.
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体WB等100微克eENZYMEIA-003-0031抗B型流感（NP）的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感（医管局）多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12（A/pintail/Alaska/102/05）（H12N5）兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4（A/environment/Maryland/1101/06）（H4N6）兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15（A/Australia/83/00（H15N8））兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16（A/Sweden/99（H16N3））兔多克隆抗体WB等100微克eENZYME
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