BSA, Biotechnology grade, 100g.Bovine serum albumin (BSA) is a large globular protein (66,000 Dal) with a good essential amino acid profile .It has been well characterized and the physical properties of this protein are wellknown ( Peters, 1975). BSA binds free fatty acids, other lipids and flavor compounds, which can alter the heat denaturation of the protein (Kinsella and Whitehead, 1992).Isolated BSA has been reported to be a very functional protein (Waniska, et.al., 1981).It is reported to partially unfold between 40 and 50oC, exposing non-polar residues on the surface and facilitating reversible protein-protein interactions.
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1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
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