Synthetic Blocking Buffer, ELISA
Cat. No. 4520
The Synthetic Blocking Buffer, ELISA is a ready-to-use solution for blocking of the remaining free binding sites after microplate coating in ELISAs. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal, improving the signal-to-noise ratio.
The Synthetic Blocking Buffer, ELISA reduces the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA.
The product is available in bulk quantities.
Specifications
- Available sizes:500 mL, 1 L, bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C
- Stability:4 years
Downloads
Download your LOT specific Certificate of Analysis (CoA)
ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
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1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
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