Product information | Protocols | References | Technical support | Reviews |
Product overview
Product name | GABA antibody - Rabbit Polyclonal antibody |
Synonyms | Anti-Gamma-Aminobutyric acid antibody |
Immunogen | Conjugated GABA |
Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with Gamma-Aminobutyric acid analogs, including Beta-Alanine and D-Alanine. |
Volume | 50µL |
Lot number | 140301 |
Storage
Form | Liquid |
Purity | Purified anti-serum |
Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
Material safety datasheet | Download MSDS |
Protocols
IF - Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
Comments | Optimal working dilutions must be determined by the end-user |
Restrictions | For research use only |
Full protocol | Download STAINperfect protocol for GABA staining |
Protocols-at-a-glance |
Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
Enregistrer
TopReferences
Product citations:
- Villamayor et al. Structural, morphometric and immunohistochemical study of the rabbit accessory olfactory bulb. Brain Struct Funct. 2019 Dec 4.
- Smits et al., Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids. bioRxiv. 2019, March 28.
- Yokoi et al. Impact of Sleep–Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons Front Neurosci. 2019 May 29.
- Traub et al. hiPS Cell-Derived Neurons for High-Throughput Screening. Methods Mol Biol. 2019;1994:243-263
Selected articles on GABA:
- Liu X, Wang Q, Haydar TF, Bordey A. Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nat Neurosci. 2005 Sep;8(9):1179-87. Epub 2005 Aug 14.
- Lawrence JJ. Cholinergic control of GABA release: emerging parallels between neocortex and hippocampus. Trends Neurosci. 2008 Jul;31(7):317-27. doi: 10.1016/j.tins.2008.03.008. Epub 2008 Jun 13.
- Baulac S, Huberfeld G, Gourfinkel-An I, Mitropoulou G, Beranger A, Prud"homme JF, Baulac M, Brice A, Bruzzone R, LeGuern E. First genetic evidence of GABA(A) receptor dysfunction in epilepsy: a mutation in the gamma2-subunit gene. Nat Genet. 2001 May;28(1):46-8.
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没有这8种氨基酸就会发生由于缺乏营养所引起的疾病;氨基酸在医药上也有很大的用途,现在手术中输液都加有各种氨基酸,使患者的抵抗力增强,同时也增加营养。随着人民生活水平的提高,人们对摄入的营养物质的要求越来越高,尤其是幼儿、青少年的健康成长,疾病患者的康复,都迫切需要高质量的营养物质,所以有效开发氨基酸食品是很有必要的。
只有9个氨基酸,分子量1KD左右,想免疫小鼠制备单抗,有没有前辈做过这方面的东西?想了一些方法,但价值不大,请前辈们指教,不甚感激。
采用OPA柱前衍生,波长是338nm
流动相A:20mmol/LNaH2PO4(用10mol/LNaOH溶液调pH至7.8±0.2);
流动相B:甲醇:乙腈:水=(45:45:10,V/V/V);
柱温:40℃;
然后进其他单标氨基酸的时候都没有问题,但是进单标胱氨酸的时候,HPLC色谱图中出了两峰,为什么会有两峰?这两峰分别是什么峰?其中一个是半胱氨酸的峰吗?用何种方法可以确定这两个分别是什么峰?
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