BiologicalActivity
Photoactivatablefluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.Non-fluorescentuntilactivatedat365nm.Photochemicalquantumyieldofuncaging=2.2%,withimprovedyielduponproteinconjugation.CompatIBLewithself-labelingtagsystems(e.g.HaloTag®andSNAPtag®).Suitableforsinglemoleculetrackingandsuperresolutionmicroscopyinlivecells,specificallylivecellsptPALMandfixedcellPALM.CanbemultiplexedwithPAJaneliaFluor®646,SE(Cat.No.6150)toperformtwo-colorsptPALMinlivecellswithactivationat405nm.Cellpermeable.Excitationmaximum=551-553nm;emissionmaximum=570-573nm.
HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.
LicensingInformation
SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus
TechnicalData
| M.Wt | 575.58 |
| Formula | C32H25N5O6 |
| Storage | Storeat-20°C |
| CASNumber | 1811539-42-0 |
| PubChemID | 126569714 |
| InChIKey | ZJZWRNGNJDEPEQ-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 5.76 | 10 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight575.58.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 1.74mL | 8.69mL | 17.37mL |
| 5mM | 0.35mL | 1.74mL | 3.47mL |
| 10mM | 0.17mL | 0.87mL | 1.74mL |
| 50mM | 0.03mL | 0.17mL | 0.35mL |
MolarityCalculator
ReconstitutionCalculator
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.
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