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Tocris/Janelia Fluor<sup>®</sup> 646, SE/6148/5/5 mg
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Tocris/Janelia Fluor® 646, SE/6148/5/5 mg
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Tocris Bioscience
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6148/5
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Description:Cellpermeablefluorescentdye;suppliedasNHSester
AlternativeNames:JF646,NHS
ChemicalName:1-[7-(1-Azetidinyl)-10-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium,innersalt
BIOLOGicalActivityTechnicalDataSolubilityCalculatorsDatasheetsReferences

BiologicalActivity

Fluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.CompatIBLewithself-labelingtagsystems(e.g.HaloTag®andSNAPtag®).Suitableforconfocalfluorescentimaging,superresolutionmicroscopy(SRM)techniquessuchasdSTORM(liveandfixedcells)andPAINTimaging.CanbemultiplexedfortwocolourimagingwithJaneliaFluor®549SE(Cat.No.6147).Cellpermeable.Excitationmaximum=646nm;emissionmaximum=664nm.Quantumyield=0.54,Extinctioncoefficient=152,000M-1cm-1;A280correctionfactoris0.19.

HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.

LicensingInformation

SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus

ApplicationofJaneliaFluor®DyesinCardiacTissue

Application of Janelia Fluor® Dyes in Cardiac Tissue

Super-resolutionimage(dSTORM)ofmuscletissuefromtheleftventricleofanadultpigheart.JF646(Cat.No.6148)wasusedtolabelcollagenVI,whichispresentonthecellplasmamembrane,andimagedwitha642nmexcitationlaser.Aclearerstructureisachievedincomparisonwiththewidefieldimage(topright).Scalebar2μm.

ImagekindlyprovidedbyProf.ChristianSoeller,UniversityofExeter;acquiredbyAlexClowsleyandAnnaMeletiou.

TechnicalData

M.Wt593.71
FormulaC33H31N3O6Si
StorageStoreat-20°C
CASNumber1811539-59-9
PubChemID124201858
InChIKeyODBCGJUBBYOJOS-UHFFFAOYSA-N
SmilesO=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CCC7)=C2

Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.

AllTocrisproductsareintendedforlaboratoryresearchuseonly.

SolubilityData

SolventMaxConc.mg/mLMaxConc.mM
Solubility
DMSO59.37100

PreparingStockSolutions

Thefollowingdataisbasedontheproductmolecularweight593.71.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.

Concentration/SolventVolume/Mass1mg5mg10mg
1mM1.68mL8.42mL16.84mL
5mM0.34mL1.68mL3.37mL
10mM0.17mL0.84mL1.68mL
50mM0.03mL0.17mL0.34mL

ProductDatasheets

CertificateofAnalysis/ProductDatasheet
SafetyDatasheet