Overview | PrinterFriendlyVersion |
Ex/Em(nm) | 544/575 |
MW | ~900 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | EnzymeDetection Phosphodiesterases |
Related | cAMPGPCRAssays BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1. Makea1mMstocksolutionbyadding500µLofDMSOintothevialof0.5µmolTAMRA-cAMPPDEIVsubstrate.
2. Make2XTAMRA-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolutionbydilute1mMTAMRA-Cyclic-3’,5’-AMPPDEIV substratestocksolutionintoyourPDEbuffer(suchas10mMTris-HCl,pH7.4,10mMMgCl2,1mMMnCl2)tomakea200-400nMsolution.Makeonlysufficientquantityneededfortheassay;storeremainingstocksolutioninaliquotsat-20°C.
3. MixequalvolumeofthePDEIVstandardsorsampleswith2XTAMRA-Cyclic-3’,5’-AMPPDEIVsubstrateassaysolution,andincubateatroomtemperatureforatleast1hour.
4. MonitorthefluorescencepolarizationatEx/Em=540/590nm(cutoff570nm).
References&Citations | PrinterFriendlyVersion |
1. AlonsoGD,SchoijetAC,TorresHN,FlawiaMM.(2006)TcPDE4,anovelmembrane-associatedcAMP-specificphosphodiesterasefromTrypanosomacruzi.MolBiochemParasitol,145,40.
2. BaderS,KortholtA,SnippeH,VanHaastertPJ.(2006)DdPDE4,anovelcAMP-specificphosphodiesteraseatthesurfaceofdictyosteliumcells.JBiolChem,281,20018.
3. BolgerGB,BaillieGS,LiX,LynchMJ,HerzykP,MohamedA,MitchellLH,McCahillA,HundsruckerC,KlussmannE,AdamsDR,HouslayMD.(2006)Scanningpeptidearrayanalysesidentifyoverlappingbindingsitesforthesignallingscaffoldproteins,beta-arrestinandRACK1,incAMP-specificphosphodiesterasePDE4D5.BiochemJ,398,23.
4. CharlieNK,ThomureAM,SchadeMA,MillerKG.(2006)TheDuncecAMPphosphodiesterasePDE-4negativelyregulatesGalpha(s)-dependentandGalpha(s)-independentcAMPpoolsintheCaenorhaBDitiseleganssynapticsignalingnetwork.Genetics,173,111.
5. Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.
6. Diaz-BenjumeaR,LaxmanS,HindsTR,BeavoJA,RasconA.(2006)CharacterizationofanovelcAMP-binding,cAMP-specificcyclicnucleotidephosphodiesterase(TcrPDEB1)fromTrypanosomacruzi.BiochemJ,399,305.
7. Gross-LangenhoffM,HofbauerK,WeberJ,SchultzA,SchultzJE.(2006)cAMPisaligandforthetandemGAFdomainofhumanphosphodiesterase10andcGMPforthetandemGAFdomainofphosphodiesterase11.JBiolChem,281,2841.
8. HanP,SonatiP,RubinC,MichaeliT.(2006)PDE7A1,acAMP-specificphosphodiesterase,inhibitscAMP-dependentproteinkinasebyadirectinteractionwithC.JBiolChem,281,15050.
9. HillEV,SheppardCL,CheungYF,GallI,KrauseE,HouslayMD.(2006)Oxidativestressemploysphosphatidylinositol3-kinaseandERKsignallingpathwaystoactivatecAMPphosphodiesterase-4D3(PDE4D3)throughmulti-sitephosphorylationatSer239andSer579.CellSignal,18,2056.
10. HsuHT,ChangYC,ChiuYN,LiuCL,ChangKJ,GuoIC.(2006)Leptininterfereswithadrenocorticotropin/3",5"-cyclicadenosinemonophosphate(cAMP)signaling,possiblythroughaJanuskinase2-phosphatidylinositol3-kinase/Akt-phosphodiesterase3-cAMPpathway,todown-regulatecholesterolside-chaincleavagecytochromeP450enzymeinhumanadrenocorticalNCI-H295cellline.JClinEndocrinolMetab,91,2761.
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想请问一下,DAPI这个染料到底有没有膜通透性,我通过百度搜索查询关于DAPI染料的,基本上是说它能透过细胞膜对活细胞和死细胞均能染上蓝色;但是也有人说DAPI只可以透过死细胞膜,不能对活细胞进行染色,用以区分活死细胞,到底哪个是对的啊,蒙了!!!!!!
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