· Plasmid Mini and Maxi Prep Methods (Gimila Lab) · Maxi-preps and all media, solutions (NWFSC)Isolation of cosmid, plasmid and P1 DNAs via diatomaceous earth-based Maxi, Midi and Mini-preps, and the non-diatomaceous earth-based Mini-prep. · Plasmid Maxiprep (Bowtell Lab) · Large scale double-stranded DNA isolation (Roe Lab)Provides protocol of isolating double-stranded DNA from plasmid or cosmid by the alkaline lysis procedure followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients and diatomaceous earth-based purification method · Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab) · CsCl Prep. of Plasmid DNA (Mike A. Dyer)This is a standard large scale prep. for plasmid DNA which gives a yield of 0.5 - 1.0 mg.
