LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!
- PeptideNKSLSSFKENEENIQC,correspondingtoaminoacidresidues84-99ofratCB1receptor(Accession P20272).Extracellular,N-terminus.
Westernblotanalysisofratbrainmembraneproteins:1. Anti-CannabinoidReceptor1(extracellular) Antibody(#ACR-001),(1:200).
2.Anti-CannabinoidReceptor1(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ExpressionofCB1receptorinmousehippocampusImmunohistochemicalstainingof mousehippocampususing Anti-CannabinoidReceptor1(extracellular) Antibody(#ACR-001),(1:100).A.CB1isdetectedinpyramidalandinfra-pyramidallayers(green).B.Stainingofinterneuronsusingmouseanti-parvalbumin(PV)antibody(red).C.ConfocalmergeofAandBdoesnotindicatethepresenceofCB1inGABAergiccells.
ExpressionofCB1receptorinrat hippocampusImmunohistochemicalstainingofrathippocampususing Anti-CannabinoidReceptor1(extracellular) Antibody(#ACR-001)(1:100).A.CB1isdetected inpyramidalandinfra-pyramidallayers(green).B.Stainingofinterneuronsusingmouseanti-parvalbumin(PV)antibody(red).C.Confocalmergeof AandB doesnot indicatethe presenceofCB1inGABAergiccells.
- Ratculturedhippocampalneurons(1:100forlivecells,1:500-1:100forfixedandpermeabilizedcells)(McDonald,N.A. etal. (2007) Mol.Pharmacol. 71, 976.).
- 1.Brooks,J.W.andFraquhar-Smith,M.A.(2003)Br.J.Anaesth.3,175.
- 2.Howlett,A.C.(2002)ProstaglandinsOtherLipidMediat.68–69,619.
- 3.Howlett,A.C.etal.(2002)Pharmacol.Rev.54,161.
- 4.Rueda,D.etal.(2000)Mol.Pharmacol.58,814.
- 5.Sarfaraz,S.etal.(2005)CancerRes.65,1635.
- 6.Casanova,M.L.etal.(2003)J.Clin.Invest.111,43.
- 7.Cota,D.etal.(2003)Int.J.Obes.Relat.Metab.Disord.27,289.
CannabinoidshavebeenusedinEasternmedicineformanyyearsaspainrelievers.1 Δ9-tetrahydrocannabinol(THC),themajorpsychoactivecompoundinmarijuanaandhashish,hasbeenshowntointeractwithtwospecificcannabinoidreceptors:cannabinoidreceptor1(CB1receptor)andcannabinoidreceptor2(CB2receptor).2 Thecannabinoidreceptorscanbedistinguishedbytheiraminoacidsequences,signalingmechanisms,andtissuedistributions.2 BothreceptorsbelongtotheG-proteincoupledreceptor(GPCR)superfamily.CB1wasshowntomodulateseveralCa2+ andK+ ionchannels.2,3
CB1isprimarilyexpressedinthecentralnervoussystem.However,expressionofCB1isalsodetectedintheperipheralterminals,innon-neuronalperipheraltissuessuchasuterus,testes,spleen,aswellasincellsoftheimmunesystem.3,4
CB1isimplicatedinmanycellularfunctionssuchasneurotransmitterrelease,painrelief,cancer,andobesity.5,6 GrowthinhibitionoftumorcellswasdemonstratedfollowingmixedCB1/CB2agoNISTtreatmentinbothprostateandnon-melanomaskincancers.5,6 ThroughtheirinteractionwithCB1,cannabinoidcompoundsstimulateappetiteforsweetsandpalatablefoodsinparticular,makingCB1anattractivetherapeutictargetforthetreatmentofobesityandeatingdisorders.7
Immuno-colocalizationofproNGFandCannabinoidReceptor1inmousehippocampaldentategyrusImmunohistochemicalstainingofimmersion-fixed,freefloatingmousebrainfrozensectionsusingGuineapigAnti-proNGFAntibody (#AGP-031),(1:300)andrabbit Anti-CannabinoidReceptor1(extracellular) Antibody(#ACR-001),(1:300).A.proNGFstaining(red)appearsinthedentategyrus(DG)granulelayer(G)andinhilarinterneurons(arrows).B.CB1staining(green)appearsinaxonalprocessessurroundingthegranulelayerandafewhilarinterneurons(arrows).C.Mergeofthetwoimagesrevealsco-localizationofproNGFandCB1inafewhilarcells.CellnucleiwerestainedwithDAPI(blue).
Anti-CannabinoidReceptor1(extracellular)Antibody(#ACR-001)isahighlyspecificantibodydirectedagainstanepitopeoftheratCB1receptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,andlivecellimagingapplications.IthasbeendesignedtorecognizeCB1inhuman,mouse,andratsamples.
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去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
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第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
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