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Novus/Recombinant Human IL-4 Protein/500 ug/204-IL-500
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Novus/Recombinant Human IL-4 Protein/500 ug/204-IL-500
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novusbio
货号 / 
204-IL-500
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RecombinantHumanIL-4ProteinSummary

AdditionalInformation
ANewrhIL-4isNowAvailable!Thenewproteinismammaliancellexpressed!
DetailsofFunctionality
MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL.ThespecificactivityofrecombinanthumanIL-4isapproximately2.9x104IU/μg,whichiscalibratedagainsthumanIL-4WHOInternationalStandard(NIBSCcode:88/656).
Source
E.coli-derivedhumanIL-4proteinHis25-Ser153,withanN-terminalMet
Accession#
P05112
N-terminalSequence
Met
Protein/PeptideType
RecombinantProteins
Gene
IL4
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
15kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
14kDa,reducingconditions
ReviewedApplications
Read2Reviewsrated5
using204-ILinthefollowingapplication:
  • CellProliferation
Publications
ReadPublicationsusing204-ILinthefollowingapplications:
  • Bioassay
    305publications
  • CellCulture
    9publications
  • ELISA(Standard)
    8publications
  • ExVivo
    1publication

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
ReconstitutionInstructions
Reconstituteat100-200μg/mLinsterilePBScontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanIL-4Protein

  • Bcellgrowthfactor1
  • BCDF
  • B-cellstimulatoryfactor1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cellstimulatoryfactor1
  • interleukin4
  • interleukin-4
  • Lymphocytestimulatoryfactor1
  • MGC79402
  • pitrakinra

Background

Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5‑7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL‑13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).

  1. Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
  2. Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
  3. Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
  4. Redfield,C.etal.(1991)Biochemistry30:11029.
  5. Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
  6. Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
  7. Mosman,T.R.etal.(1987)J.Immunol.138:1813.
  8. Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
  9. Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
  10. Paludan,S.R.(1998)Scand.J.Immunol.48:459.
  11. Corthay,A.(2006)Scand.J.Immunol.64:93.
  12. Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
  13. Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
  14. Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.

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前段时间见到论坛有人找一些病毒的抗原,没找到原帖子,先发个发布帖吧
有一下一些病毒抗原蛋白提供。
InfectiousDiseases
-CMVAntigens
-EBVAntigens
-EncephalitisAntigens
-HepatitisAntigens
-HIV&HTLVAntigens
-HSVAntigens
-RubellaAntigens
-SARSAntigens
-ToxoplasmaAntigens
-TreponemaAntigens
-VZVAntigens
1.机体免疫功能检查
主要是中度以上细胞免疫缺陷包括:CD4+T淋巴细胞耗竭,外周血淋巴细胞显著减少,CD4<200/μl,CD4/CD8<1.0,(正常人为1.25~2.1),迟发型变态反应皮试阴性,有丝分裂原刺激反应低下。NK细胞活性下降。
2.各种致病性感染的病原体检查
如用PCR方法检测相关病原体,恶性肿瘤的组织病理学检查。
3.HIV抗体检测
采用酶联免疫吸附法、明胶颗粒凝集试验、免疫荧光检测法、免疫印迹检测法、放射免疫沉淀法等,其中前三项常用于筛选试验,后二者用于确证试验。
4.PCR技术检测HIV病毒。
流行性感冒病毒抗原的两种变异形成式
我没有做过ELISA,请教一下各位,用全病毒做抗原包板做间接ELISA,对病毒纯度和病毒量的要求怎样?多谢!
艾滋病毒
由上可知,HBV的抗原有3种:表面抗原(HBsAg)、核心抗原(HBcAg)和e抗原(HBeAg)。表面抗原大量存在于感染者血液中,是HBV感染以及检测的主要标志。它具有抗原性,可诱导机体产生特异保护性的抗-HBs,也是制备疫苗的最主要成分。
核心抗原由183个或185个氨基酸组成,高度磷酸化,是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面,被表面抗原覆盖,故不易在血循环中检出。核心抗原具有强免疫原性,可诱导很强的体液免疫和细胞免疫,刺激机体产生抗-HBc。
e抗原为可溶性蛋白质,传染性强,游离存在于血液中,虽然很早就被发现,在病理上认为是HBV复制以具有强感染性的一个指标,但其功能尚不清楚。抗-HBe的出现,是预后良好的征象。向左转|向右转
方法一:可以解除root。
1.在手机上下载360手机卫士。

2.下载完成手机卫士之后,在手机中安装360手机卫士。在手机中打开360手机卫士并授予手机卫士 Root 权限,如图所示。
3.在手机卫士主界面打开“应用工具”选项,进入后找到“一键 Root 工具”并打开。如果手机已经获取 Root 权限,手机卫士会提示已经手机获取 Root 权限,可以解除 Root 了。

4.点击“解除 Root”按钮之后,360 Root 工具就会给出提示,“注意!解除 Root 之后将永久失去 Root 权限,并且可能无法重新获取!因此若非送修等特殊需要,不建议解除......”确认后点击“解除 Root”按钮即可解除 Root 权限。

方法二:恢复手机的出厂设置。
1.在待机页面下,点击【应用程序】。
2.点击【设定】。

3.向上滑动手机屏幕,点击【重置】。

4.点击【恢复出厂设定】。

5.点击【重置设备】。

6.点击【全部删除】。

7.完成操作后,待手机自动重启后,手机就成功恢复出厂设定了。
TGEV用PK-15细胞培养,PEDV用VERO细胞培养,准备将这两种病毒纯化,用于包被ELISA板,但不知道应该如何纯化这两种病毒。特向大家请教!
请教大家EB病毒抗原,或者破伤风类毒素TT(tetanustoxoid)怎么订到,本人做实验快疯了。
两个概念:
1、存在于细胞外环境时,不显复制活性,但保持感染活性,是病毒体或病毒颗粒形式。
2、抗原,是指能够刺激机体产生(特异性)免疫应答,并能与免疫应答产物抗体和致敏淋巴细胞在体外结合,发生免疫效应(特异性反应)的物质
4、说出HBV抗原-抗体系统组成及其临床意义。P159-160

①乙型肝炎表面抗原—抗体系统(HBsAg/抗—HBs);

②乙型肝炎核心抗原—抗体系统(HBcAg/抗—HBc):

③乙型肝炎e抗原—抗体系统(HBeAg);

④乙型肝炎Dane颗粒抗原—抗体系统

⑤乙型肝炎δ抗原—抗体系统(δ/抗—δ)。

临床意义  1.HBsAg:血清中检测到HBsAg ,表示体内感染了HBV,因而是一种特异性标志。HBsAg阳性见于:①急性乙型肝炎的潜伏期或急性期(大多短期阳性);②HBV致的慢性肝病、迁延性和慢性活动性肝炎、肝炎后肝硬化或原发性肝癌等。③无症状携带者 

 2.抗HBs:表示曾感染过HBV,不论临床上有无肝炎症状表现,均已得到恢复,并且对HBV有一定的免疫力。 

3.HBcAg与抗HBc:由于 HBcAg主要存在于肝细胞核内,并仅存在于Dane颗粒中。因此,对病人血清不能检测HBcAg,而测抗HBc。血清内抗HBc阳性反映:①新近有过HBV感染;②体内有HBV增殖;③有助于诊断急性或慢性乙型肝炎,特别是少数病例就诊时已处于急性恢复期早期,HBsAg已从血中消失,此时血中仅有抗HBc存在,因此,对恢复期患者可作病因追索。 

 4.HBcAg和抗HBe:HBcAg的存在常表示病人血液有感染性。 HBcAg阳性揭示病人肝脏可能有慢性损害,对预后判断有一定帮助。抗HBe阳性对病人可能有一定的保护力。展开
这里有个填空题,不知道如何下手,麻烦各位帮忙看看,多谢了

流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.

我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?

多谢各位啊!
新年快乐!
万事如意!