Description
Ultrapure dGTP supplied as sodium salt in purified water (pH 8.5).
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
DNA amplification
cDNA synthesis
Storage
-20°C for 24 months
Ultrapure
Greater than 99% purity confirmed by HPLC.
Highly Stable
The purity of SMOBIO’s dNTP remained greater than 99% after one week storage at 25°C.
Functionality
SMOBIO’s dNTP are optimal performance for general PCR (A), long range PCR (B), RT-PCR (C), and real-time PCR (D).
Specification
Product Name | dATP Solution - Sodium Salt | dTTP Solution - Sodium Salt | dCTP Solution - Sodium Salt | dGTP Solution - Sodium Salt | dUTP Solution - Sodium Salt |
Cat. No. | CD3000 | CD4000 | CD5000 | CD6000 | CD7000 |
Nomenclature | 2’-Deoxyadenosine 5’-triphosphate | 2’-Deoxythymidine 5’-triphosphate | 2’-Deoxycytidine 5’-triphosphate | 2’-Deoxyguanosine 5’-triphosphate | 2’-Deoxyuridine 5’-triphosphate |
Formula (Anion) | C10H13N5O12P3 | C10H14N2O14P3 | C9H13N3O13P3 | C10H13N5O13P3 | C9H12N2O14P3 |
Storage | at -20 °C | at -20 °C | at -20 °C | at -20 °C | at -20 °C |
Stability | 24 months | 24 months | 24 months | 24 months | 24 months |
Appearance | Colorless | Colorless | Colorless | Colorless | Colorless |
Concentration | 100-110mM | 100-110mM | 100-110mM | 100-110mM | 100-110mM |
Purity (HPLC) | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% | ≧ 99% |
A250/A260 | 0.78±0.02 | 0.64±0.02 | 0.82±0.02 | 1.15±0.03 | 0.74±0.03 |
A280/A260 | 0.15±0.01 | 0.74±0.02 | 0.97±0.02 | 0.67±0.02 | 0.38±0.02 |
A290/A260 | N/A | 0.24±0.02 | 0.30±0.02 | 0.28±0.02 | N/A |
pH (4°C) | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 | 8.5±0.1 |
Cation (Mg2+) | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM | ≦ 5 mM |
Phosphate (PO43-) | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM | ≦ 2 mM |
Contamination with human DNA (18S rRNA) or bacterial DNA (16S rRNA) | Negative | Negative | Negative | Negative | Negative |
Proteases, DNases, RNases, or Nicking Activity | Negative | Negative | Negative | Negative | Negative |
Manual
Manual_CD6000_SMOChem™ dGTP Solution - Sodium Salt
SDS
SDS_CD6000
Flyer
Purity of dNTPs- A Critical Factor for Successful PCR
SMOBIO"s dNTPs- Specification, Purity and Functionality
Product Description | Size | Cat. No. |
dATP Solution - Sodium Salt (100 mM) | 25 ml | CD3000 |
100 ml | CD3001 | |
dTTP Solution - Sodium Salt (100 mM) | 25 ml | CD4000 |
100 ml | CD4001 | |
dCTP Solution - Sodium Salt (100 mM) | 25 ml | CD5000 |
100 ml | CD5001 | |
dGTP Solution - Sodium Salt (100 mM) | 25 ml | CD6000 |
100 ml | CD6001 | |
dUTP Solution - Sodium Salt (100 mM) | 25 ml | CD7000 |
100 ml | CD7001 |
Deoxynucleotide (dNTP) Mix, 10 mM each (40 mM total) | 200 µl | CD1010 |
200 µl x 5 | CD1011 |
Deoxynucleotide (dNTP) Mix, 25 mM each (100 mM total) | 500 µl | CD1020 |
500 µl x 6 | CD1021 |
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DNA连接酶主要是连接DNA片段之间的磷酸二酯键最初从原核生物(大肠杆菌)分离得到的.现在生物基因工程主要是从T4噬菌体中分离得到的,
酶对所作用的底物有严格的选择性。一种酶仅能作用于一种物质,或一类分子结构相似的物质,促其进行一定的化学反应,产生一定的反应产物,这种选择性作用称为酶的专一性。
连接酶通常是包括“连接酶”这个字,就如DNA连接酶是将脱氧核糖核酸(DNA)片段连接。其他普遍的名称包括“合成酶”,因为这些酶是用作合成新的分子,或当它们是将二氧化碳加入一个分子时则称为“羧化酶”。
菌体构建时,目的基因连接在T载体上,测序也正确,但是将目的基因还有载体分别双酶切后总是连接不上,将连接后产物跑核酸胶,什么也没有,不知道是什么原因?我的目的基因浓度为9ng/ul,载体回收后的浓度为6ng/ul,是因为浓度太低的原因吗?还有就是连接有目的基因的T载体双酶切后出现了三条带,这个又是什么原因呢?希望得到解答
大家有用过Invitrogen的T4连接酶吗?说明书上是23-26度连接,一般的连接酶不都是16度吗?应该用多少度呢?另外,说明书上还说连接后为了达到更好的转化效率,应将连接反应液至少稀释5倍再转化,是这样吗?谢谢大家帮忙啊
连接酶有T4噬菌体DNA连接酶、T4噬菌体RNA连接酶、大肠埃希菌DNA连接酶等。DNA连接酶可连接平端,也连接粘端。反应需有Mg2+和ATP存在,pH7.5-7.6。最适温度37℃,30℃以下活性明显下降,但考虑到被连接DNa 的稳定性和粘性末端的退火温度,一般平端连接用20-25℃,粘端连接用12℃左右。
聚合酶有DNA聚合酶(以DNA为模板合成DNA大肠埃希菌DNA聚合酶Ⅰ,大肠埃希菌DNA聚合酶Ⅰ大片段(Klenow大片段),T4或T7噬菌体DNA聚合酶等);RNA聚合酶(以DNA为模板合成RNA,T7或T3噬菌体RNA聚合酶);逆转录酶(以RNA为模板合成DNA,除RNA病毒中发现外,发现大肠埃希菌DNA聚合酶Ⅰ和Taq DNA聚合酶都有逆转录活性)。
大肠杆菌DNA聚合酶Ⅰ具有5′→3′聚合酶活性和5′→3′,3′→5′外切酶活性。Klenow片段是DNA聚合酶Ⅰ被枯草杆菌酶作用产生的一个大片段,有5′→3′聚合酶和3′→5′外切酶活性,无5′→3′外切酶活性。可用于缺口翻译(Nick translation)法标记核酸,也可用于DNA序列测定,修补DNA链等。向左转|向右转
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