Reagents:Lysisbuffer25mMTris-HCl,pH7.45mMEDTA1mMATP20µg/mlCLAP1mMPMSFBufferA10mMMgCl20.2MTris-HCl,pH7.40.2%TritonX-100BufferB0.2Msodiumacetate,pH5.00.2%TritonX-100BufferC0.2MTris-HCl,pH7.40.2%TritonX-100Isolatingcellularenzyme1)Grow5x108cellsunderregulargrowthconditions.2)PelletcellsandwashonetimewithicecoldPBS.3)ResUSPendpelletin10mloficecol 查看更多>

（一）原理酶免疫电镜技术是利用酶的高效率的催化作用对其底物的反应形成不同的电子密度，借助于电子显微镜观察，证明酶的存在，从而对抗原进行定位。（二）材料与试剂1．PBS液取NaCl8.5g，Na2HPO40.85g，KH2PO40.54g，加水至1000ml即可。2．DAB溶液取5mgDAB(3、3二氨基� 查看更多>

MSP,macrophage-stimulatingprotein,actsthroughthetransmembranereceptorkinaseRON(Stkinmice)toplayaroleininflammationandtheresponsetotissueinjury.MSPissecretedbytheliverintothebloodaspro-MSP,aninactiveprecursorform.MSPisrelatedtoclottingfactorsthatareactivatedbyproteolyticcleavageandpro-MSPisitselfalsoactivatedbycleavagetoMSP.BindingofactivatedMSPhete 查看更多>

武田旗下Ninlaro（ixazomib）胶囊日前在美国上市，这是上市的首款也是唯一一款蛋白酶体抑制剂。Ninlaro旨在与来那度胺和地塞米松合并用于既往至少接受过一种治疗的多发性骨髓瘤患者。「目前随着Ninlaro在美国的上市，我们将之前的广泛研究变成了现实。除了临床研 查看更多>

AmpC酶是AmpCβ内酰胺酶的简称。是由肠杆菌科细菌或和绿脓假单胞菌的染色体或质粒介导产生的一类β内酰胺酶,属β内酰胺酶Ambler分子结构分类法中的C类和BushJacobyMedeiros功能分类法中第一群，即作用于头孢菌素、且不被克拉维酸所抑制的β内酰胺酶。故AmpC酶又称作为� 查看更多>

Contributor:SupryaJayadevDate:Oct.20,1994Reagents:2NmethanolicNaOH2NHClBligh&Dyerextraction1)Pelletapproximately1X107cells.2)ResUSPendpelletin3mlCHCl3:CH3OH(1:2)andvortexhard.3)Add0.8mlH2O,vortexhardandallowsolutiontositatroomtemperatureforatleast30minutes.4)Pelletcellulardebrisbyspinningat3,000rpm(inthetabletopcentrifuge)for5minutes.5)Transfer 查看更多>

Theeukaryoticgenomeispackagedbyhistoneandnonhistoneproteinstoformchromatin.Theassemblyofnucleosomesaswellascompactionofnucleosomalarraysintohigher-orderchromatinstructurescreateahighlyrestrictiveenvironmentfornuclearprocessesthatrequireaccesstoDNA.ThepackagingofeukaryoticDNAintonucleosomesinhibitstheaccessoffactorstoDNAandthusresultsintherepression 查看更多>

Duringthisweek,youwillanalyzethefattyacidcompositionoftheindividuallipidfractionsrecoveredfromtheTLCplate.Gaschromatographyisaverysensitivemethodfortheseparationandquantificationofchemicals,anditisperfectfortheanalysisoffattyacidcomponents.Likeinanyotherchromatographictechnique,separationofcompoundsdependsontheirpartitionbetweenastationaryandamobil 查看更多>

TypicaltransformationresultsThefollowingtransformationswereperformedatthesametimeusingthemultiwelltransformationprotocol.SimilaramountsofPCRproductwereusedforeach.ThepicturesdemonstratethedegreeofsuccessandvariABIlitythatcanbeattained.Theplateswereincubatedfor6daysat30°Cbeforebeingphotographed.Thehaploidplateshowsthemanysmallerbackgroundcolonies(1 查看更多>

QuickYeastDNAPrep:IsolationofTotalDNA(genomicandplasmid)LindaHoskins,HahnLab10/16/98Growa5mlYPDO/Ncultureinoculatedwithasingleyeastcolonyat30deg.Transferculturetoasmall13x100glasstube.Spindowncells2min.intabletopcentrifuge.Pouroffsupernatant.Washcellswith5mlH2O.Spindowncells2min.Pouroffsupernatant.Cellpelletcanbestoredat-20deg.ResUSPendcellsin500ul 查看更多>