Technical Documents
Description
Glen-Pak™ DNA and RNA cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5" terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak DNA Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak DNA 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak DNA 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak DNA resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo. Scale suggestions for the Glen-Pak DNA product line are shown below:
Glen-Pak DNA Product | Catalog Number | Synthesis Scale Compatibility | |
Glen-Pak DNA 50mg Purification Cartridge | 60-5000-96 | 0 nmole - 200 nmole | |
Glen-Pak DNA Purification Cartridge | 60-5100-XX and 60-5200-XX | 10 nmole - 1.0 µmole | |
Glen-Pak DNA Cartridge 3G | 60-5300-01 | 5 µmole - 20 µmole | |
Glen-Pak DNA 30 mg 96-Well Plate | 60-5400-01 | 10 nmole - 50 nmole | |
Glen-Pak DNA 3mg 384-Well Plate | 60-5500-xx | up to 20 nmoles |
A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.This booklet is available online at: https://www.glenresearch.com/media/productattach/g/l/glen-pak_2.9_1.pdf.
Details
Specifications | |
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Storage | Controlled room temperature |
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。
mRNA 数量不详,根据转录数量,加工数量各不相同,故无法得知。
用醌指纹法描述微生物群落的参数[7]有:(1)醌的类型和不同类型的醌的数目;(2)占优势的醌及其摩尔分数含量;(3)总的泛醌和总的甲基萘醌的摩尔分数之比;(4)醌的多样性和均匀性;(5)醌的总量等。对两个不同的群落,由上述分析所得数据可以计算出另一个参数____非相似性指数(D),用于定量比较两个群落结构的差异。
醌指纹法具有简单快速的特点,近几年来广泛用于各种环境微生物样品(如土壤,活性污泥和其它水生环境群落)的分析。
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
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