Highlights
- Quick recovery of ultra-pure DNA from agarose gels.
- Column design permits DNA elution at high concentrations into minimal volumes.
- Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Description
Applicable For | Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
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Elution Volume | ≥ 6 µl of DNA Elution Buffer |
Equipment | Microcentrifuge |
Purity | A260/A280 >1.8, A260/A230>1.8 |
Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
Size Range | 50 bp to 23 kb |
Yield | Typically, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70% |
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: Is it possible to use higher binding capacity columns for the Zymoclean workflow?
Zymoclean has been validated for use with Zymo-Spin IIC and IIN columns. Other columns with higher binding capacity have not been validated by Zymo Research but have successfully been used by users.
Q3: Are the columns and DNA Wash buffer interchangeable with the DCC-5 Kit?
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Q4: Should more Agarose Dissolving Buffer be used for higher % gels?
Add 4 volumes of Agarose Dissolving Buffer for agarose gels >2% and ensure the gel slice has been fully dissolved.
Q5: Can Zymoclean be used for samples in solution (without agarose gel)?
No, high efficiency recovery is not guaranteed and the success of the purification can be variable.
Q6: Can Zymoclean be used to recover ssDNA?
If only isolating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Recovery Kit. With some modification of the Zymoclean Gel DNA Recovery protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Q7: What is the maximum weight of gel slice that can be used?
Up to 400 mg of agarose gel.
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Always had good results with your products.”
-Simin H. (University of California, Irvine)
Read MoreCat # | Name | Size | Price | |
---|---|---|---|---|
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
C1004-50 | Zymo-Spin IC Columns | 50 Pack | $53.00 | |
C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 | |
D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 |
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我在一些文献上看到说,要加入tRNA as carrier,但是这句话不好理解
有没有人用过,跟我讲讲tRNA到底有什么用
多谢指教
1、测浓度和纯度,是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足,就不需要纯化,否则需要进一步纯化。
不过根据经验,一般都不需要,我们实验室用的是GNT系列的试剂盒,提取结果就直接进入下游实验了,效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器(主要指线粒体和叶绿体)中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌)、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
向左转|向右转
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
目的掌握RNA提取的基本技术,了解RNA提取过程中的各种注意事项。二、原理RNA提取是分子生物学实验中难度较大的实验技术。RNA提取和DNA提取有类似的地方,因为它们都是核酸,都具有较好的水溶性。提取RNA首先破碎细胞,然后用提取液将RNA溶出,反复抽提去除蛋白质 ……
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