DescriptionViPrimePLUS One Step Taq RT-qPCR Master Mix is next generation master mix designed for one step realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step Taq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS One Step Taq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RTqPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step real time RT-qPCR reaction set up
- Equipped with thermostable M-MULV enzyme
- Good buffer system for excellent amplification efficiency
- High sensitivity detection
- Optimal performance for highly sensitive and specific RT-qPCR reaction
- Compatible with most of the real-time PCR platforms
Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step Taq RTqPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
Product Description | Compatible Hardware |
QLMM13ViPrimePLUS One Step Taq RT-qPCR Master Mix | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
QLMM13-LRViPrimePLUS One Step Taq RT-qPCR Master Mix with Low ROX | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
QLMM13-RViPrimePLUS One Step Taq RT-qPCR Master Mix with ROX | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
Catalog No | Description | Pack Size |
QLMM13 | ViPrimePLUS One Step Taq RT-qPCR MasterMix | 150 reactions |
QLMM13-LR | ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX | 150 reactions |
QLMM13-R | ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX | 150 reactions |
DownloadManual
ViPrimePLUS One Step Taq RT-qPCR MasterMix ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX
PublicationThis Product Has Been Used In:Attaran, B., Falsafi, T. (2017).). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。
mRNA 数量不详,根据转录数量,加工数量各不相同,故无法得知。
用醌指纹法描述微生物群落的参数[7]有:(1)醌的类型和不同类型的醌的数目;(2)占优势的醌及其摩尔分数含量;(3)总的泛醌和总的甲基萘醌的摩尔分数之比;(4)醌的多样性和均匀性;(5)醌的总量等。对两个不同的群落,由上述分析所得数据可以计算出另一个参数____非相似性指数(D),用于定量比较两个群落结构的差异。
醌指纹法具有简单快速的特点,近几年来广泛用于各种环境微生物样品(如土壤,活性污泥和其它水生环境群落)的分析。
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
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