Description:
KAPASingle-IndexedAdapterKit,SetB(30µM)KAPAmRNAHyperPrepKits
Single-DayRNA
TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.
Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:
- single-daylibraryconstruction,inclusiveofmRNAcapture
- reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
- flexibleinputof50ng–1µgtotalRNA*
- maintainover99%strandspecificity*
- KAPAPureBeadsincludedforreactionpurifications
KAPARNAHyperPrepKits
KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.
NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Sequencewhatmatters
- WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievesuperiorcoverageuniformity
- Obtainmoreuniformdistributionofreadsacrosstranscripts
- ImprovecoverageofdifficultGC-richtranscripts
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- Geneexpressionanalysis
- Detectionofgenefusions,isoforms,andotherstructuralvariants
- Noveltranscriptidentification,includingnoncodingtranscripts
- SNVdiscovery
- Wholetranscriptome(KAPARNAHyperPrepKitsonly)
- Targetedtranscriptome(KAPARNAHyperPrepKitsonly)
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageEnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)
KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.
Specifications
- SpecDescription
- CompatibilityPlatformIlluminaHiSeq,NextSeq,andMiSeq
- LibraryTypeRNA
- StartingMaterialRNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA
- StartingMaterialRNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。
mRNA 数量不详,根据转录数量,加工数量各不相同,故无法得知。
用醌指纹法描述微生物群落的参数[7]有:(1)醌的类型和不同类型的醌的数目;(2)占优势的醌及其摩尔分数含量;(3)总的泛醌和总的甲基萘醌的摩尔分数之比;(4)醌的多样性和均匀性;(5)醌的总量等。对两个不同的群落,由上述分析所得数据可以计算出另一个参数____非相似性指数(D),用于定量比较两个群落结构的差异。
醌指纹法具有简单快速的特点,近几年来广泛用于各种环境微生物样品(如土壤,活性污泥和其它水生环境群落)的分析。
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
暂无品牌问答