MouseEsophagus TotalRNAisextractedfromfreshlyharvestedtissuesofsinglehealthynormaldonorusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodwhichallowstherapidisolationoftotalRNAincludingmicroRNAs. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA,preciselyquantified,andstoredat-80oC.
QualityControl: TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: RNAisidealforNorthernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com
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校准品:公司指定用校准某检测系统(仪器+试剂+程序)考虑具基质效应情况赋予校准品校准值校准品必须专用于某检测系统北京标准物质网:www.biaowu.com
Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM:标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度(BS,NISTBASARMI等)(ISO Guide 30-1992)
Reference Materials(RM)
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. (ISO Guide 30-1992)
照品RM:种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植(ISO Guide 30-1992)
我做实验的药物很难买到标准品,但现在有粗品,自己在提纯,想问下各位大牛,在没有标准品的情况下,怎么测提纯后该物质的纯度。谢谢
各们前辈,初涉诊断行业,开发PCR诊断试剂,求问微生物标准品或者质控血清(比如支原体、衣原体、金葡菌等)应该到哪里购买啊?中检所和卫生部临检中心好像都没有。谢谢!
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