Product Specifications:
Item# 63001: Recombinant West Nile Envelope E Protein (E.coli)
Concentration: See vial
Mass/vial: See vial
Diluent: 50mM Tris, 0.02% SDS, pH8
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Applications: ELISA, Western Blot, West Nile Diagnostics
Description: Synthetic West Nile full length envelope E protein DNA sequence, expressed as a his-tag protein in the E.coli Expression System.
Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 56.1kD
Specificity: This protein binds to murine monoclonal antibodies- structural epitopes, and to West Nile virus converted horse sera as determined by Elisa and Western blot assays.
Biological Activity: Not Determined.
Application and Instructions for use:
Recommended concentrations for use are approximate values. Elisa is performed in 1-2uglml protein range for titeringvirus converted horse serum polyclonal antibodies.
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抗原与其所诱导产生的抗体或致敏淋巴细胞特异性结合的能力。抗原性的强弱与抗原分子的大小、化学成分、抗原决定簇的结构、抗原与被免疫动物亲缘关系的远近等有密切关系。通常认为抗原的分子量愈大、化学组成愈复杂、立体结构愈完整以及与被免疫动物的亲缘关系愈远,则抗原性愈强。抗原的物理状态也对抗原性发生影响,例如蛋白质,聚合状态的比单体的抗原性强,一般球形分子的比纤维形分子的抗原性强。抗原加入佐剂改变物理状态后,抗原性也得到增强。例如,分子量高达10万的明胶由于缺乏苯环氨基酸,稳定性较差,在进入机体后容易被酶降解成低分子物质,如果加入少量酪氨酸(苯环氨基酸),就能增强其抗原性。
中文名:抗原性
外文名:antigenicity
别称:免疫反应性
抗原性介绍:流感病毒按抗原性
看了百科以后依然不太明白,语言通俗一点最好。谢谢。
另一类抗原是手术移植的器官,如异体移植的肾、肝或其他结构。因为这个器官并不是受植者本人的结构,会被人体免疫系统认为是入侵人体的抗原,并对其进行攻击,这就是所谓的排斥反应。
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