吉玛公司microRNAs定量PCR检测解决方案
Hairpin-itTMmiRNAsqPCRQuantitationAssay原理 |
Hairpin-itTMmiRNAsqPCRQuatitationAssay包括两步 |
1. Stem-loopRT 2.Real-timePCR |
A. Stem-loopRTprimer与miroRNA分子的3’端结合,然后用逆转录酶将RNA逆转录成cDNA; B. 将得到RT产物与miroRNA特异引物以及荧光标记的MolecularBeaconprobes一起进行优化的real-timePCR反应。 |
Hairpin-itTMmiRNAsqPCRQuantitationAssay原理
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Hairpin-itMicroRNAsQuantitationPCR的特点
- 高度特异,特异性的Stem-loopRTprimer与miR-specificMolecularBeaconprobe双重保证,确保反应不受其前体及其他因素干扰;序列高度同源的miRNAs也可精确区分;
- 超宽的定量线性范围和高度检测灵敏度,从几个拷贝一直到几万个拷贝,定量线性范围跨越7个数量级;
- 样品消耗量少,仅需1-10ngtotalRNA;
- 适用的样品范围广,totalRNA、细胞裂解物以及纯化的RNA都可以用于qRT-PCR的模板,即使基因组DNA的污染也不会干扰miRNA的定量;
- 金牌质量的miRNA标准合成物,即可精确用来作标准曲线绝对定量之用,又可用来作相对定量的阳性对照
- 操作快速简单,可实现RT与PCR反应一步完成;
- 重复性好,经过吉玛公司经验丰富的技术人员严格的质量检验。
Hairpin-itTMmiRNAsqPCRQuantitationKit |
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目录号 | 规格 | 价格 | |||
QPM-010 | 100rxns | ¥1,000 | |||
QPM-011 | 200rxns | ¥1,600 | |||
QPM-012 | 300rxns | ¥2,500 | |||
QPM-013 | 500rxns | ¥3,000 | |||
| □产品特点 Ø高度特异性的miRNAHairpinprimer; Ø只对成熟MicroRNAs进行定量,不受其前体干扰;序列高度同源的miRNAs也可精确区分; Ø灵敏度极高,仅需1-10ng的totalRNA或同等物; Ø超宽的定量线性范围,可跨越7个数量级,从几个拷贝一直到几百万个拷贝; Ø可实现对每个细胞所含目标miRNA分子进行准确定量;
□适用的荧光定量PCR仪器 ABIPRISM7000/7300/7500/7900 MJ-opticon、Opticon2及Chromo4系列、MX3000p/4000 Bio-RadIQ系列、LightCycler、Rotogene3000、FTC-2000
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□试剂盒组成(40µl×100rxns)
Real-timePCRMasterMix(5×)* 1ml×1支 miRNAHairpin-primerSet(10µM) 100µl×1支 miRNARTprimer(10µM) 15µl×1支 TaqDNApolymerase(5U/µl) 50µl×1支 SyntheticmiR-16Standard 1支 1XRNADilutionbuffer 1ml×2支
*内含dNTPMixture,Mg2+,SYBRGreen1等。
□试剂盒的保存 -20℃避光保存,避免反复冻融。保质期6个月。 |
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RegularlyStoredmicroRNAsqPCRQuantificationKits | |||||
AssayName | MaturemiRNA Sequence | Human | Mouse | Rat | *价格(QuantitationKit) |
h-let-7a | ugagguaguagguuguauaguu | Yes | Yes | Yes | ¥1,000 |
h-let-7b | ugagguaguagguugugugguu | Yes | Yes | Yes | ¥1,000 |
h-let-7d | agagguaguagguugcauagu | Yes | Yes | Yes | ¥1,000 |
h-let-7e | ugagguaggagguuguauagu | Yes | Yes | Yes | ¥1,000 |
h-let-7g | ugagguaguaguuuguacagu | Yes | Yes |
| ¥1,000 |
h-let-7i | ugagguaguaguuugugcu | Yes | Yes | Yes | ¥1,000 |
h-miR-9 | ucuuugguuaucuagcuguauga | Yes | Yes | Yes | ¥1,000 |
h-miR-9* | uaaagcuagauaaccgaaagu | Yes | Yes |
| ¥1,000 |
h-miR-10a | uacccuguagauccgaauuugug | Yes | Yes | Yes | ¥1,000 |
h-miR-15a | uagcagcacauaaugguuugug | Yes | Yes |
| ¥1,000 |
h-miR-15b | uagcagcacaucaugguuuaca | Yes | Yes | Yes | ¥1,000 |
h-miR-16 | uagcagcacguaaauauuggcg | Yes | Yes | Yes | ¥1,000 |
h-miR-17-3p | acugcagugaaggcacuugu | Yes |
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| ¥1,000 |
h-miR-17-5p | caaagugcuuacagugcagguagu | Yes | Yes | Yes | ¥1,000 |
h-miR-19a | ugugcaaaucuaugcaaaacuga | Yes | Yes | Yes | ¥1,000 |
h-miR-20 | uaaagugcuuauagugcaggua | Yes |
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| ¥1,000 |
h-miR-21 | uagcuuaucagacugauguuga | Yes | Yes | Yes | ¥1,000 |
h-miR-23a | aucacauugccagggauuucc | Yes | Yes | Yes | ¥1,000 |
h-miR-23b | aucacauugccagggauuaccac | Yes | Yes | Yes | ¥1,000 |
h-miR-25 | cauugcacuugucucggucuga | Yes | Yes | Yes | ¥1,000 |
h-miR-26a | uucaaguaauccaggauaggcu | Yes | Yes | Yes | ¥1,000 |
h-miR-26b | uucaaguaauucaggauaggu | Yes |
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| ¥1,000 |
h-miR-27a | uucacaguggcuaaguuccgcc | Yes |
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| ¥1,000 |
h-miR-27b | uucacaguggcuaaguucug | Yes | Yes | Yes | ¥1,000 |
h-miR-28 | aaggagcucacagucuauugag | Yes | Yes | Yes | ¥1,000 |
h-miR-29a | cuagcaccaucugaaaucgguu | Yes | Yes | Yes | ¥1,000 |
h-miR-29b | uagcaccauuugaaaucagu | Yes |
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| ¥1,000 |
h-miR-29c | uagcaccauuugaaaucgguua | Yes | Yes | Yes | ¥1,000 |
h-miR-30a-3p | cuuucagucggauguuugcagc | Yes | Yes | Yes | ¥1,000 |
h-miR-30b | uguaaacauccuacacucagc | Yes | Yes | Yes | ¥1,000 |
h-miR-30c | uguaaacauccuacacucucagc | Yes | Yes | Yes | ¥1,000 |
h-miR-30d | uguaaacauccccgacuggaag | Yes | Yes | Yes | ¥1,000 |
h-miR-30b | uguaaacauccuacacucagc | Yes | Yes | Yes | ¥1,000 |
h-miR-30e | uguaaacauccuugacugga | Yes | Yes | Yes | ¥1,000 |
h-miR-31 | ggcaagaugcuggcauagcug | Yes |
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| ¥1,000 |
*注:该价格为QuantitationKit100rxns规格试剂盒报价,其他规格试剂盒与Hairpin-itTMmiR-16qPCRQuantitationKit相应规格试剂盒价格相同; 由于MicroRNAs数量众多,吉玛公司可以为其他miRNAs序列提供Hairpin-itTMmiRNAsQuantitationAssay定制服务,具体定制流程请咨询吉玛公司 |