Source | M.W. | 320.26 | CAS No. | 146986-50-7 | |
---|---|---|---|---|---|
Structural Info | |||||
Formulation | Powder | ||||
Reconstitution | Before reconstitution, we recommend abrief spin to drive down any material dislodged from the bottom of thetube. The compound is soluble in DMSO. | ||||
Stability | The powder is stable for at least 2year if stored at -20 degree C. Thedissolved compound is stable for at least 1 month at 4 degree C, but shouldbe stored in aliquots at -20 degree C for longer term. Protect from light. | ||||
Purity | Greater than 99% as determined by LC/MS analysis.LC/MS and/or NMR data available upon request. | ||||
Biological Activity | Tested active for culturing human iPS cells in serum-free medium on Matrigel coated plates. | ||||
Country of Origin | USA |
Y-27632, (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexane-carboxamid-2HCl, is a Rho-associated kinase (ROCK) inhibitor. Y27632 has been shown to prevent apoptosisas well as enhance the survival and cloning efficiency of dissociated humanembryonic stem (ES) cells without affecting their pluripotency. Ref: Watanabe, K., etal. (2007). A ROCK inhibitor permits survival of dissociated human embryonicstem cells. Nat Biotechnol 25: 681-686.
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《血管紧张素转换酶抑制剂在心血管病中应用的中国专家共识》.PDF(242.69k)
1、测定酶比活力:底物需要过量么?测定时间多长?是否可以加入过量的底物,然后测定3min吸光度的增加值,从吸光度的变化值计算比活力。
2、在酶抑制剂筛选的过程中,是否需要保证底物过量?还是要水浴一定时间让反应完全?我看到文献说用终浓度为0.1μmol/mL的底物,终浓度为45U/ml的酶,我想问,假设总体积为1ml,那么终浓度为45U/ml的酶岂不是每分钟能转化4.8μmol的底物?那么0.1μmol/mL的底物不就几秒钟就反应完了?那么怎么测定初速度?
1.对于抑制剂筛选工作(求ic50)是不是体系内酶与底物的量(底物应该是过量的)对实验结果影响不大。
2.如果要求算Km值,是不是需要知道反应产物的绝对量。反应时间文献上都是5分钟,反应速度就用反应产物量除以反应时间即可。
3.酶是进口分装的,规格5U,一次用不完,用PBS稀释后如何保存
谢谢
2、可逆抑制剂:包括
a、竞争性抑制剂,抑制剂与底物竞争性结合酶反应中心,使Km增大,而Vmax不变 b、非竞争性抑制剂,酶与抑制剂结合后还能与底物结合,但活性降低,使Vmax减小,而Km不变
c、反竞争性抑制剂,酶只能与底物结合后才能与抑制剂结合,Vmax与Km都减小
可逆抑制剂可用透析等方法除去,使酶恢复作用
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