Description
Product Highlights
- Efficient – effectively inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C
- High-quality - certified free of contaminating DNases, RNases, phosphatases and nickases for improved sample security
- Robust – active up to 55 oC across a wide range of DTT concentrations and pH
- Flexible – compatible with all common reverse transcriptases, bacterial polymerases and thermostable polymerases
Product Description
Ribonucleases (RNases) are ubiquitous and can be introduced into experiments in many ways: for example co-purification during RNA isolation, carryover from bare hands and pipette tips. This RNase contamination can often go unnoticed. RiboSafe RNase Inhibitor is ideal for RNA-sensitive applications such as RT-qPCR as even a small amount of RNase can be detrimental to the final experimental outcome.
RiboSafe RNase Inhibitor is a highly efficient inhibitor of a broad spectrum of eukaryotic RNases and shows no inhibition of polymerase or reverse transcriptase activity, so can be used in cDNA synthesis or one-step RT-qPCR reactions.
RiboSafe RNase Inhibitor is tested for, purity (run on SDS-PAGE gel), presence and absence of endonucleases, nickases and exonucleases, thermostability, inhibition at different pH levels and activity in the presence or absence of DTT. This quality control allows RiboSafe RNase Inhibitor to be used in highly-sensitive techniques such as single-cell RT-PCR, in vitro RNA synthesis and in vitro translation.
Applications
- RNA purification
- cDNA synthesis
- One-step RT-PCR
- One-step RT-qPCR
- In vitro transcription/translation
- RNA Sequencing
- RNase protection assays
- Enzymatic RNA labeling reaction
dNTP Guide
Download the dNTP Guide with detailed product descriptions and performance data to help you choose the best product for your researchebiomall.com
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《血管紧张素转换酶抑制剂在心血管病中应用的中国专家共识》.PDF(242.69k)
1、测定酶比活力:底物需要过量么?测定时间多长?是否可以加入过量的底物,然后测定3min吸光度的增加值,从吸光度的变化值计算比活力。
2、在酶抑制剂筛选的过程中,是否需要保证底物过量?还是要水浴一定时间让反应完全?我看到文献说用终浓度为0.1μmol/mL的底物,终浓度为45U/ml的酶,我想问,假设总体积为1ml,那么终浓度为45U/ml的酶岂不是每分钟能转化4.8μmol的底物?那么0.1μmol/mL的底物不就几秒钟就反应完了?那么怎么测定初速度?
1.对于抑制剂筛选工作(求ic50)是不是体系内酶与底物的量(底物应该是过量的)对实验结果影响不大。
2.如果要求算Km值,是不是需要知道反应产物的绝对量。反应时间文献上都是5分钟,反应速度就用反应产物量除以反应时间即可。
3.酶是进口分装的,规格5U,一次用不完,用PBS稀释后如何保存
谢谢
2、可逆抑制剂:包括
a、竞争性抑制剂,抑制剂与底物竞争性结合酶反应中心,使Km增大,而Vmax不变 b、非竞争性抑制剂,酶与抑制剂结合后还能与底物结合,但活性降低,使Vmax减小,而Km不变
c、反竞争性抑制剂,酶只能与底物结合后才能与抑制剂结合,Vmax与Km都减小
可逆抑制剂可用透析等方法除去,使酶恢复作用
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