I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting mouse IL-1α. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate.
Pre-coated plates reduce assay time and minimize variability.
Mouse IL-1α ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.
Analyte Information
IL-1αInterleukin-1α (IL-1α, also known as hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α and Interleukin-1 alpha) is a proinflammatory cytokine and inducer of acute phase responses. IL-1α is produced primarily by monocytes, macrophages, and neutrophil after induction by bacterial product such as LPS.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Application: |
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Documentation: | Datasheet/ProtocolSDS |
Contents: |
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Size: | 2 plates |
Other formats: | Click to view all Mouse IL-1α products |
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请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。
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