The monkey IL-13 ELISA development kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of monkey IL-13 in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP and ELISA standard. Plates and substrate are not included.
The monoclonal antibodies in this kit react with human IL-13 and cross-react with IL-13 from non-human primates. The ELISA standard supplied is recombinant human IL-13. See the Human/NHP cross-reactivity table.
Monkey IL-13 ELISA development kits are also available with Streptavidin-ALP. See Related products.
Serum/Plasma samplesAnalysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-13Interleukin-13 (IL-13, IL13) is produced by TH2-type cells as well as mast cells and NK cells in response to activation. It regulates the secretion of IgE by B cells and the TH2-type cell development and suppresses inflammatory responses by regulation of macrophage function.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Application: |
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Reactivity: |
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Standard range: | 3-300 pg/ml |
Documentation: | Datasheet/Protocol |
Contents: |
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Size: | for 20 plates |
Other formats: | Click to view all NHP/Human IL-13 products |
Save time andreduce variability with thecompleteand pre-coated ELISAPRO kit | |
Very easy to use ELISA kit. |
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请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。
暂无品牌问答