This monoclonal antibody enables sensitive and specific detection of rat IgG2a in e.g. ELISA and ELISpot.
The antibody is specific for rat IgG2a. There is no cross-reactivity (<0.1%) with rat IgG1, IgG2b, IgG2c, IgA, IgM, IgE, or with IgG from mouse, cow, rabbit, horse, goat, human, monkey, cat or dog.
We recommend MT171A1 as capture antibody in rat IgG2a ELISA and ELISpot in combination with biotinylated detection antibody MT168B1. See Related products.
Analyte Information
IgG2aIgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3.The IgG2a allele is expressed in e.g. Balb/c mice.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Bulk OrdersUpon request, Mabtech"s antibodies are available in unconjugated format and may be purchased in bulk. Please contact Mabtech directly for further details.
Application: |
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Format: | Purified |
Specificity: | Rat IgG2a. No cross-reactivity (<0.1%) with rat IgG1, IgG2b, IgG2c, IgA, IgM, IgE or with IgG from mouse, cow, rabbit, horse, goat, human, monkey, cat or dog. |
Isotype: | Mouse IgG1 |
Documentation: | DatasheetSDS |
Contents: |
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Size: | 1 mg |
Other formats: | Click to view all Rat IgG2a products |
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请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
尤其是获得SCI文献引用的产品,
绝对ok的
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。
暂无品牌问答