The products listed on this page are being phased out. Replacement products are already available:
We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taq is available as individual components, in a hot-start formulation, or as a complete kit.
The products listed on this page are being phased out. Replacement products are already available:We recommend replacing Taq full DNA polymerase with the newer TaKaRa Taq DNA polymerase, which is a recombinant version of the thermostable, full-length Taq. Takara Taqpolymerase is available as individual components, in a hot-start formulation, or as a complete kit.
Taqfull DNA polymerase is an ultra-pure, highly efficient, full-length, recombinant version of the Thermus aquaticus (Taq) YT1 DNA Polymerase (94 kD) (Engelke et al. 1990). This enzyme possesses two important catalytic activities: a 5"→3" polymerase activity, and a double-strand-specific 5"→3" exonuclease activity. Like other full-length Taq DNA Polymerases, this enzyme lacks 3"→5" exonuclease (proofreading) activity. This enzyme is suitable for any general PCR amplification procedure. It amplifies even rare targets from bacterial and plasmid DNA, cDNA, and complex genomic DNA.
Taqfull DNA polymerase is available in several formats:
- Individual components of polymerase enzyme mix (Cat. # 639233 and 639234)—separate tubes ofTaqfull DNA polymerase and 10X buffer
- Taq full DNA polymerase in a complete kit (Cat. # 639235)—includes Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
- A hot-start polymerase formulation in a complete kit (Cat. # 639231)—includes hot-start Taq, 10X buffer, MgCl2, dNTPs, control genomic DNA template, and primer mix
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谢谢谢
只要引物设计的可以在基因组上做,那么理论上是没问题的!
只需要用RT-PCR荧光定量试剂盒里面的第二个,专门用来做Real-timePCR的那些试剂就可以了!
如果要是直接注射,就不知道了
试剂盒里有详细的说明书,告诉你样品需要多少量,每个试剂需要加入多少量,和详细的实验步骤,一般买来就可以用,不用人教。
所以你问一个样需要多少量是没法回答的,测定过程是要加很多种试剂的。
定性ELISA只能粗略表示样本待测物含量在某个特定值以上或以下,定性ELISA通常设置阳性对照(P)、和阴性对照(N),结果分别用“阴性”和“阳性”表示。ELISA定性测定的“阴性”和“阳性”的判断标准是试剂盒所确定的阳性判断值,即cut-off值。
定量ELISA是通过一系列不同已知浓度的标准品所对应的OD值做出标准曲线,然后将样本的OD值代入标准曲线,计算出样本中待测物的含量。百特纯大分子Meretciel的定量ELISA试剂盒还可以。
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