Description
The RB6-8C5 monoclonal antibody reacts with the mouse Ly-6G (also known as Gr-1). The Ly-6G protein is a myeloid differentiation antigen of 21-25 kDa, expressed in a regulated manner by the myeloid lineage in the bone marrow, where the level of antigen expression is correlated with the granulocyte maturation and differentiation. In the bone marrow, the antigen is not expressed by the erythroid cells.
From the peripheric cells, RB6-8C5 binds with monocytes, neutrophils and eosinophils.
As a marker for the mouse monocytes, macrophages and granulocytes, the RB6-8C5 antibody is usually combined with M1/70, a macrophage labeling antibody (Anti-CD11b), for phenotypic analysis.
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1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?
在兔子上研究肝脏脂代谢,不知道什么细胞株合适,自己培养的担心不靠谱,不知道有没有做过这方面的,给提一些意见,感谢!
我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?
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