Antigen Information
- IGA
- IGE
- IGG2A
- IGHG1
- IGM
Assay Format
- Mouse
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
- Sandwich-based
- Semi-Quantitative
Product Specifications
Product Feature
- One step mouse monoclonal antibody isotyping
- Use only 1-2 µl sample
- The whole experiment can be done within 1 hour
- Sandwich based technology for high specificity and sensitivity
- Low system CV with high reproducibility
- High throughput sample processing
- Qualitative visual inspection or semi-quantitative result
- Processed slides can be stored for years without signal decay
Target Name
Target Species
Application Notes
- Mouse Ig Rapid Isotyping Glass Slide(s)
- Sample Diluent
- 20X Wash Buffer
- Labeled Detection Antibody
- Slide Washer/Dryer
- 96-well Plate
- Manual
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
- Dry the glass slide
- Mix samples with detection antibodies (in 96-well plate)
- Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
- Shao W., Zhang C., Liu E., et al. Identification of Liver Epithelial Cell-derived Ig Expression in ? chain-deficient mice. Sci Rep. 2016; 6: 23669. Published online 2016 Mar 29. doi: 10.1038/srep23669Species: MouseSample type: Tissue Lysate
- Groscurth S., Muller B., Schwan B., et al. Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices. Biomacromolecules. 2012 Oct 8;13(10):3076-86. doi: 10.1021/bm3008499Species: MouseSample type: Conditioned Media
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1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?
我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?
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