ContentsPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiSECTION I: B-CELL EPITOPE MAPPING1 What Is a B-Cell Epitope?. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Marc H.V. Van RegenmortelA: WHOLE ANTIGEN METHODS2 Structural Basis of Antibody–Antigen Interactions . . . . . . . . . . . . . . . . . . . . . . . . 23Eric J. Sundberg3 Epitope Mapping of Antibody–Antigen Complexesby Nuclear Magnetic Resonance Spectroscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 37Osnat Rosen and Jacob Anglister4 A Solid-Phase Mutual Inhibition Assay with Labeled Antigen . . . . . . . . . . . . . . . . 59Masahide Kuroki5 Epitope Mapping by Surface Plasmon Resonance . . . . . . . . . . . . . . . . . . . . . . . . . 67P r S fsten6 Proteolytic Fragmentation for Epitope Mapping . . . . . . . . . . . . . . . . . . . . . . . . . . 77Maria R. Mazzoni, Francesca Porchia, and Heidi E. Hamm7 Epitope Mapping by Proteolysis of Antigen–Antibody Complexes . . . . . . . . . . . . 87Suraj Dhungana, Jason G. Williams, Michael B. Fessler, and Kenneth B. Tomer8 Identifying Residues in Antigenic Determinantsby Chemical Modification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103Howard M. Reisner and Roger L. Lundblad9 Epitope Mapping by Differential Chemical Modification of Antigens . . . . . . . . . . 119Suraj Dhungana, Michael B. Fessler, and Kenneth B. TomerB: PEPTIDE LIBRARY APPROACHES10 Linear B-Cell Epitope Mapping Using Enzyme-LinkedImmunosorbent Assay for Libraries of Overlapping Synthetic Peptides . . . . . . . . . 137Michael W. Heuzenroeder, Mary D. Barton, Thiru Vanniasinkam,and Tongted Phumoonna11 Antibody Epitope Mapping Using SPOT Peptide Arrays . . . . . . . . . . . . . . . . . . 145Ulrich Reineke and Robert Sabat12 Peptide Microarrays for Profiling of Modification State-Specific Antibodies . . . . . 169Johannes Zerweck, Antonia Masch, and Mike Schutkowski13 Epitope Mapping Using Phage Display Peptide Libraries . . . . . . . . . . . . . . . . . . . 181Volker B ttger and Angelika B ttger14 Antibody Epitope Mapping Using De Novo GeneratedSynthetic Peptide Libraries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203Ulrich Reineke15 Antibody Specificity Profiling on Functional Protein Microarrays . . . . . . . . . . . . . 213Dawn R. Mattoon and Barry Schweitzer16 Peptide Microarrays for Determination of Cross-Reactivity . . . . . . . . . . . . . . . . . . 225Alexandra ThieleC: PROFILING OF ANTIBODY SIGNATURES IN BIOLOGICAL FLUIDS17 Epitope Mapping Using Randomly Generated Peptide Libraries . . . . . . . . . . . . . . 237Juliane Bongartz , Nicole Bruni, and Michal Or-Guil18 Probing the Epitope Signatures of IgG Antibodies in HumanSerum from Patients with Autoimmune Disease . . . . . . . . . . . . . . . . . . . . . . . . . . 247Peter Lorenz, Michael Kreutzer, Johannes Zerweck, Mike Schutkowski,and Hans-Jürgen Thiesen19 Microarrayed Allergen Molecules for Diagnostics of Allergy . . . . . . . . . . . . . . . . . 259Jing Lin , Ludmilla Bardina, and Wayne G. Shreffler20 Monitoring B Cell Response to ImmunoselectedPhage-Displayed Peptides by Microarrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 273Lina Cekaite, Eiving Hovig, and Mouldy SioudD: ANTIGEN EXPRESSED FROM RECOMBINANT DNA21 Epitope Mapping Using Homolog-Scanning Mutagenesis . . . . . . . . . . . . . . . . . . 289Lin-Fa Wang22 Epitope Mapping by Region-Specified PCR-Mutagenesis . . . . . . . . . . . . . . . . . . . 305Tsutomu Mikawa, Masayuki lkeda, and Takehiko Shibata23 Epitope Mapping Using Phage-Display Random Fragment Libraries . . . . . . . . . . 315Lin-Fa Wang and Meng YuE: B-CELL EPITOPE PREDICTION24 Prediction of Linear B-cell Epitopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335Ulf ReimerSECTION II: T-CELL EPITOPE MAPPING25 Molecular Recognition of Diverse Ligands by T-Cell Receptors . . . . . . . . . . . . . . 347Eric J. Sundberg26 Identification of Human MHC Class I Binding Peptidesusing the iTOPIA Epitope Discovery System . . . . . . . . . . . . . . . . . . . . . . . . . . . 361Markus Wulf, Petra Hoehn, and Peter Trinder27 T-Cell Epitope Mapping in Mycobacterium tuberculosisUsing PepMixes Created by Micro-Scale SPOT Synthesis . . . . . . . . . . . . . . . . . 369Marisa Frieder and David M. Lewinsohn28 High-Throughput T-Cell Epitope DiscoveryThrough MHC Peptide Exchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 383Sine Reker Hadrup, Mireille Toebes, Boris Rodenko, Arnold H. Bakke,David A. Egan, Huib Ovaa, and Ton N.M. Schumacher29 T-Cell Epitope Processing (The Epitope Flanking Regions Matter). . . . . . . . . . . . 407Alejandra Nacarino Martinez, Stefan Tenzer, and Hansj rg Schild30 Identification of MHC Class II Binding Peptides: Microarrayand Soluble MHC Class II Molecules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 417Simani Gaseitsiwe and Markus J. Maeurer31 T-Cell Epitope Mapping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 427Raija K.S. Ahmed and Markus J. Maeurer32 Identification and Validation of T-Cell EpitopesUsing the IFN-γ EliSpot Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 439Markus Wulf Petra Hoehn, and Peter TrinderIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 447http://g.zhubajie.com/urllink.php?id=1052162633wnltnf43jkotyo