产品说明
DescriptionManualS.D.SDetection Principle-Bacstain- Bacterial Viability Detection Kits are a series of products for fluorescent double-staining of bacteria. By combining different types of fluorescent stain, staining images can be acquired for each index (membrane impairment, respiratory activity, and esterase activity). Bacterial viability is generally assessed by colony formation in the nutrient-agar medium. However, this requires a long culturing time, and it is hard to recognize the growth of viable but nonculturable (VNC) bacteria. However, fluorescent staining does not require bacterial culture and enables viability assessment by rapid and simple protocols.-Bacstain- Bacterial Viability Detection Kit – DAPI/PI uses bacterial membrane damage as an index. DAPI is a minor groove binder specific to the AT sequence of DNA and permeates into bacteria to stain nucleic acids regardless of membrane damage. PI is a parallel intercalator into the DNA double helix that stains nucleic acid; it passes only through damaged bacterial membranes. Thus, this kit can be used to measure the ratio of membrane-damaged bacteria to total bacteria on the analysis of the fluorescent images from each stain.Simple ProcedureDouble-staining of Staphylococcus aureus (a Gram-positive bacterium)Quantitative analysis-Bacstain- Bacterial Viability Detection Kit – DAPI/PI (BS08) and –CTC/DAPI (BS09) were used to evaluate the effect of benzalkonium chloride on S. aureus (Gram-positive bacteria) by fluorescent imaging. The images were quantified by ImageJ and show the correlation with drug concentration.The results showed that the effects of benzalkonium chloride on the respiratory activity and membrane damage of S. aureus varied greatly depending on the indicator detected.The evaluation of multiple indicators will contribute to improving the reliability of drug efficacy measurements by providing a multidimensional view of the activity of the bacterium that would be missed by a single indicator.Related Product InformationProductSizeProduct Code-Bacstain- CFDA solution100 assaysBS03-Bacstain- DAPI solution100 assaysBS04-Bacstain- AO solution100 assaysBS05-Bacstain- PI solution100 assaysBS07-Bacstain- CTC Rapid Staining Kit (for Flow cytometry)100 assaysBS01-Bacstain- CTC Rapid Staining Kit (for Microscopy)100 assaysBS02Related Categories All Products Bacterial Staining Cellular Assays
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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