产品说明
DescriptionReferencesS.D.SProduct DescriptionCarboxy-PTIO is a stable, water-soluble organic radical that reacts with NO to form NO2· This reaction can be monitored by electron spin resonance (ESR). NO is an unstable molecule and has a complex reaction cascade for its metabolism in biological systems. Rapidly generated NO-related metabolites carry out various physiological activities. Commonly used NO scavengers such as hemoglobin trap NO; they also trap NOS inhibitors such as arginine derivatives. These NO scavengers also quench all other NO-related metabolites at the same time. In contrast, Carboxy-PTIO does not dramatically affect other NO-related product systems because it transforms NO to NO2, which is a metabolite of NO. Thus, Carboxy-PTIO can be used to investigate the effects of NO separately from its downstream metabolites. Dr. Akaike and others showed that Carboxy-PTIO suppresses relaxation of the rat aorta ring, which is induced by acetylcholine, twice as effectively as NG-nitroarginine. Dr. Yoshida and others reported that downstream metabolites of NO, generated by treatment with Carboxy-PTIO, have an increased antiviral activity compared to NO alone. The NO metabolites play important roles in biological systems; therefore, they should be investigated separately from NO.Reaction of NO quenching1. E. F. Ullman, et al., Studies of Stable Free Radicals. X. Nitronyl Nitroxide Monoradicals and Biradicals as Possible Small Molecule Spin Labels. J Am Chem Soc. 1972;94:7049-7059.2. Y. Miura, et al., Polymers Containing Stable Free Raficals, 5. Preparation of a Polymer Containing Imidazoline 3-Oxide 1-Oxyl Groups. Macromol Chem Phys. 1973;172:233-236.3. K. Inoue, et al., Magnetic Properties of the Crystals of p-(1-Oxyl-3-Oxide-4, 4, 5, 5-Tetramethyl-2-Imidazolin-2-Yl)Benzoic acid and Its Alkali Metal Salts. Chem Phys Lett. 1993;207:551-555.4. T. Akaike, et al., Antagonistic Action of Imidazolineoxyl N-Oxides Against Endothelium-Derived Relaxing Factor/NO Through a Radical Reaction. Biochemistry. 1993;32:827-832.5. J. Joseph, et al., Trapping of Nitric Oxide by Nitronyl Nitroxides: an Electron Spin Resonance Investigation. Biochem Biophys Res Commun. 1993;192:926-934.6. M. Yoshida, et al., Therapeutic Effects of Imidazolineoxyl N-Oxide Against Endotoxin Shock Through Its Direct Nitric Oxide-scavenging Activity. Biochem Biophys Res Commun. 1994;202:923-930.7. T. Az-Ma, et al., Reaction Between Imidazolineoxil N-Oxide(Carboxy-PTIO) and Nitric Oxide Released from Cultured Endothelial Cells:Quantitative Measurement of Nitric Oxide by ESR Spectrometry. Life Sci. 1994;54:PL185-PL190.8. H. Maeda, et al., Multiple Functions of Nitric Oxide in Pathophysiology and Microbiology: Analysis by a New Nitric Oxide Scavenger. J Leukoc Biol. 1994;56:588-592.9. T. Akaike, et al., Quantitation of Nitric Oxide Using 2-Phenyl-4, 4, 5, 5-Tetramethylimidazoline-1-Oxyl 3-Oxide(PTIO). Methods Enzymol. 1996;268:211-221.10. S. Satoh, et al., NO Donors Stimulate Noradrenaline Release from Rat Hippocampus in a Calmodulin-dependent Manner in the Presence of LCysteine. J Cell Physiol. 1996;169:87-96.11. D. C. Hooper, et al., Prevention of Experimental Allergic Encephalomyelitis by Targeting Nitric Oxide and Peroxynitrite: Implications for the Treatment of Multiple Sclerosis. PNAS. 1997;94:2528-2533.12. S. Pfeiffer, et al., Interference of carboxy-PTIO with Nitric-Oxide and Peroxynitrite-Mediated Reactions. Free Radic Biol Med. 1997;22:787-794.Related Categories Oxidative Stress Array
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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