产品说明
DescriptionApplicationReferencesDataManualS.D.SRequired Equipment and Materials10 μl, 1000 μl pipettes, incubator, Microscope (blue excitation filter and red emission filter) or flow cytometer (488 nm blue laser)Fig. 1 Cell staining mechanismStaining procedure1. Allow CFDA solution to stand at room temperature for 30 minutes to thaw. Solution should be protected from light.2. Resuspend the organism with an appropriate buffer (phosphate buffer, saline, etc.) a) and adjust the number of cells to 106 cells/mL(flow cytometry) or 108-109 cells/mL(microscopy).3. Add CFDA solution into the microbial cell suspension and vortex gently to mix. Use 5 μl for flow cytometry and 15 μl for microscopy analysis. The maximum wavelengths of the dye are 493 nm for excitation and 515 nm for emission.4. Incubate the microbial cell at 37ºC for 5 minutes b).5. Fix the microbial cell by addition of formaldehyde (1-4% final concentration).6. Remove the buffer by filtration or centrifugation, and resuspend with buffer.7. Analyze the stained-cells by a flow cytometer or a microscope.a) Gram-negative bacteria tend to exhibit lower fluorescence intensity than Gram-positive bacteria because of their cell structure (outer membrane impedes penetration of CFDA). For the staining of Gram-negative bacteria, use 0.1 M phosphate buffer, 0.9 M NaCl, 0.5 mM EDTA, pH 8.5.b) If CFDA staining is not sufficient with 5 minutes of incubation, increase the incubation time.1. N. Yamaguchi, et al., Flow cytometric analysis of bacterial respiratory enzymatic activity in the natural aquatic environment. J Appl Microbiol. 1997;83:43-52.2. M. Kawai, et al., Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process. J Appl Microbiol. 1999;86:496-504.Staining DataFig. 2 B. cereus stained with CFDA and DAPI (left). S. epidermidis stained with CFDA and PI (right).Related Categories Microbial Viability Assay
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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