产品说明
DescriptionReferencesS.D.SReaction SchemeProduct DescriptionMTT is slightly soluble in methanol, but has very low solubility in ethanol. It barely dissolves in ether, acetone, or ethyl acetate. MTT is utilized for cell viability detection. Since MTT has a positive charge, it can pass through a cell membrane and is reduced by mitochondria to form a purple color formazan dye. Organic solvent is requried to dissovle MTT formazan dyes.1. T. F. Slater, et al., Studies on Succinate-tetrazolium Reductase Systems III. Points of Coupling of Four Different Tetrazolium Salts. Biochim Biophys Acta. 1963;77:383-393.2. T. Mosmann, et al., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J Immunol Methods. 1983;65:55-63.3. H. Tada, et al., An Improved Colorimetric Assay for Interleukin 2. J Immunol Methods. 1986;93:157-165.4. M. C. Alley, et al., Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Res. 1988;48:589-601.5. E. Aoyama, et al., Determination of Selenium by Flow Injection Analysis Based on the Selenium(III)-Catalyzed Reduction of 3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2H Tetrazolium Bromide. Anal Sci. 1991;7:103-107.6. H. Yamaue, et al., Chemosensitivity Testing with Highly Purified Fresh Human Tumor Cells with the MTT Colorimetric Assay. Eur J Cancer. 1991;27:1258-1263.Related Categories Cell Proliferation / Cytotoxicity Redox Dyes
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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