产品说明
DescriptionApplicationReferencesDataS.D.SProduct DescriptionCFSE is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins (Fig. 1). Hydrolyzed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively. CFSE-stained cells are shown in Fig. 2.Staining Procedure1.Prepare 1 mM CFSE solution with DMSO. Dilute it to prepare 10-50 μM CFSE solution with PBS or an appropriate buffer.2.Add CFSE solution with 1/10 of the volume of cell culture medium to the cell culture.3.Incubate the cell at 37ºC for 15 to 30 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 490 nm excitation and 530 nm emission filters.1. M. Bronner-Fraser, Alterations in Neural Crest Migration by a Monoclonal Antibody That Affects Cell Adhesion. J Cell Biol. 1985;101:610-617.2. A. Nose, et al., A Novel Cadherin Cell Adhesion Molecule:Its Expression Patterns Associated WithImplantation and Organogenesis of Mouse Embryos. J Cell Biol. 1986;103:2649-2658.3. S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.4. C. K. Raymond, et al., Molecular Analysis of the Yeast VPS3 Gene and the Role of Its Product in Vacuolar Protein Sorting and Vacuolar Segregation during the Cell Cycle. J Cell Biol. 1990;111:877-892.5. G. Radcliff, et al., Quantification of Effector/Target Conjugation Involving Natural Killer(NK) or Lymphokine Activated Killer(LAK) Cells by Two-color Flow Cytometry. J Immunol Methods. 1991;139:281-292.6. S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.7. L. S. D. Clerck, et al., Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. J Immunol Methods. 1994;172:115-124.Fig. 2 Cell staining with CFSECell type: HeLaRelated Categories Cell Staining
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
文章排行榜
1
株式会社 同仁化学研究所
2
Blockade of L-type Ca2+ channel attenuates doxorubicin...
3
Systembio(SBI) Exosome-depleted FBS 去外泌体血清(无外泌体血清)
4
超氧化物歧化酶SOD活性检测试剂盒
5
让我们共同打造－“BIOSITE Triage®”主题活动(04-12-1)
6
绵羊、狗和SD大鼠的麻醉方法
7
众美生物代理品牌及产品线介绍
8
医院岗位绩效管理与成本精准管控课程通知
9
2008第十三届广州全国药品保健品展览会
10
2009医学前沿论坛暨第十一届全国肿瘤药理与化疗学术会议
微信公众号
手机扫码，关注动态